6r7m
From Proteopedia
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==Tobacco Mosaic Virus (TMV)== | ==Tobacco Mosaic Virus (TMV)== | ||
| - | < | + | <SX load='6r7m' size='340' side='right' viewer='molstar' caption='[[6r7m]], [[Resolution|resolution]] 1.92Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6r7m]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Tobacco_mosaic_virus Tobacco mosaic virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R7M OCA]. For a <b>guided tour on the structure components</b> use [http:// | + | <table><tr><td colspan='2'>[[6r7m]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Tobacco_mosaic_virus Tobacco mosaic virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R7M OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6R7M FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http:// | + | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6r7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r7m OCA], [http://pdbe.org/6r7m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6r7m RCSB], [http://www.ebi.ac.uk/pdbsum/6r7m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6r7m ProSAT]</span></td></tr> |
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | High-resolution structural information is essential to understand protein function. Protein-structure determination needs a considerable amount of protein, which can be challenging to produce, often involving harsh and lengthy procedures. In contrast, the several thousand to a few million protein particles required for structure determination by cryogenic electron microscopy (cryo-EM) can be provided by miniaturized systems. Here, we present a microfluidic method for the rapid isolation of a target protein and its direct preparation for cryo-EM. Less than 1 muL of cell lysate is required as starting material to solve the atomic structure of the untagged, endogenous human 20S proteasome. Our work paves the way for high-throughput structure determination of proteins from minimal amounts of cell lysate and opens more opportunities for the isolation of sensitive, endogenous protein complexes. | ||
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| + | Microfluidic protein isolation and sample preparation for high-resolution cryo-EM.,Schmidli C, Albiez S, Rima L, Righetto R, Mohammed I, Oliva P, Kovacik L, Stahlberg H, Braun T Proc Natl Acad Sci U S A. 2019 Jul 10. pii: 1907214116. doi:, 10.1073/pnas.1907214116. PMID:31292253<ref>PMID:31292253</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6r7m" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
| - | </ | + | </SX> |
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Tobacco mosaic virus]] | [[Category: Tobacco mosaic virus]] | ||
Current revision
Tobacco Mosaic Virus (TMV)
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Categories: Large Structures | Tobacco mosaic virus | Albiez, S | Braun, T | Kovacik, L | Mohammed, I | Oliva, P | Righetto, R | Rima, L | Schmidli, C | Stahlberg, H | Cryo-em | Cryowriter | Microfluidic | Mosaic | Tmv | Tobacco | Virus
