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| | ==Pseudorabies virus (PrV) nuclear egress complex proteins fitted as a hexameric lattice into a sub-tomogram average derived from focused- ion beam milled lamellae electron cryo-microscopic data== | | ==Pseudorabies virus (PrV) nuclear egress complex proteins fitted as a hexameric lattice into a sub-tomogram average derived from focused- ion beam milled lamellae electron cryo-microscopic data== |
| - | <StructureSection load='5fki' size='340' side='right'caption='[[5fki]], [[Resolution|resolution]] 35.00Å' scene=''> | + | <SX load='5fki' size='340' side='right' viewer='molstar' caption='[[5fki]], [[Resolution|resolution]] 35.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5fki]] is a 84 chain structure with sequence from [http://en.wikipedia.org/wiki/Alphaherpesvirus_pseudorabies_virus_prv Alphaherpesvirus pseudorabies virus prv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FKI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FKI FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5fki]] is a 84 chain structure with sequence from [https://en.wikipedia.org/wiki/Suid_alphaherpesvirus_1 Suid alphaherpesvirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FKI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FKI FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 35Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">UL31 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10345 Alphaherpesvirus pseudorabies virus PRV]), UL34 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10345 Alphaherpesvirus pseudorabies virus PRV])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5fki FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fki OCA], [https://pdbe.org/5fki PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5fki RCSB], [https://www.ebi.ac.uk/pdbsum/5fki PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5fki ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5fki FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5fki OCA], [http://pdbe.org/5fki PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5fki RCSB], [http://www.ebi.ac.uk/pdbsum/5fki PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5fki ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/G3G955_SUHV G3G955_SUHV] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| - | </StructureSection> | + | </SX> |
| - | [[Category: Alphaherpesvirus pseudorabies virus prv]]
| + | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Antonin, W]] | + | [[Category: Suid alphaherpesvirus 1]] |
| - | [[Category: Dent, K C]] | + | [[Category: Antonin W]] |
| - | [[Category: Gruenewald, K]] | + | [[Category: Dent KC]] |
| - | [[Category: Hagen, C]] | + | [[Category: Gruenewald K]] |
| - | [[Category: Mettenleiter, T C]] | + | [[Category: Hagen C]] |
| - | [[Category: Mordehai, T Zeev Ben]]
| + | [[Category: Mettenleiter TC]] |
| - | [[Category: Vasishtan, D]] | + | [[Category: Vasishtan D]] |
| - | [[Category: Alpha-herpesvirinae]] | + | [[Category: Zeev Ben Mordehai T]] |
| - | [[Category: Fib-sem]]
| + | |
| - | [[Category: Focused ion beam milling]]
| + | |
| - | [[Category: Herpesvirus simplex]]
| + | |
| - | [[Category: Hsv-1]]
| + | |
| - | [[Category: Inner nuclear membrane]]
| + | |
| - | [[Category: Nuclear egress complex]]
| + | |
| - | [[Category: Nuclear envelope]]
| + | |
| - | [[Category: Nucleo-cytoplasmic transport]]
| + | |
| - | [[Category: Nucleoplasmic reticulum]]
| + | |
| - | [[Category: Prv]]
| + | |
| - | [[Category: Pseudorabies virus]]
| + | |
| - | [[Category: Ryofib]]
| + | |
| - | [[Category: Vesicle transport]]
| + | |
| - | [[Category: Viral protein]]
| + | |
| Structural highlights
Function
G3G955_SUHV
Publication Abstract from PubMed
Although nucleo-cytoplasmic transport is typically mediated through nuclear pore complexes, herpesvirus capsids exit the nucleus via a unique vesicular pathway. Together, the conserved herpesvirus proteins pUL31 and pUL34 form the heterodimeric nuclear egress complex (NEC), which, in turn, mediates the formation of tight-fitting membrane vesicles around capsids at the inner nuclear membrane. Here, we present the crystal structure of the pseudorabies virus NEC. The structure revealed that a zinc finger motif in pUL31 and an extensive interaction network between the two proteins stabilize the complex. Comprehensive mutational analyses, characterized both in situ and in vitro, indicated that the interaction network is not redundant but rather complementary. Fitting of the NEC crystal structure into the recently determined cryoEM-derived hexagonal lattice, formed in situ by pUL31 and pUL34, provided details on the molecular basis of NEC coat formation and inner nuclear membrane remodeling.
Crystal Structure of the Herpesvirus Nuclear Egress Complex Provides Insights into Inner Nuclear Membrane Remodeling.,Zeev-Ben-Mordehai T, Weberruss M, Lorenz M, Cheleski J, Hellberg T, Whittle C, El Omari K, Vasishtan D, Dent KC, Harlos K, Franzke K, Hagen C, Klupp BG, Antonin W, Mettenleiter TC, Grunewald K Cell Rep. 2015 Dec 29;13(12):2645-52. doi: 10.1016/j.celrep.2015.11.008. Epub, 2015 Dec 17. PMID:26711332[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Zeev-Ben-Mordehai T, Weberruss M, Lorenz M, Cheleski J, Hellberg T, Whittle C, El Omari K, Vasishtan D, Dent KC, Harlos K, Franzke K, Hagen C, Klupp BG, Antonin W, Mettenleiter TC, Grunewald K. Crystal Structure of the Herpesvirus Nuclear Egress Complex Provides Insights into Inner Nuclear Membrane Remodeling. Cell Rep. 2015 Dec 29;13(12):2645-52. doi: 10.1016/j.celrep.2015.11.008. Epub, 2015 Dec 17. PMID:26711332 doi:http://dx.doi.org/10.1016/j.celrep.2015.11.008
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