5aju

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of ligand-free phosphoribohydroxylase lonely guy from Claviceps purpurea in complex with phosphoribose

Structural highlights

5aju is a 1 chain structure with sequence from Claviceps purpurea. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.5Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

M1VUY5_CLAP2

Publication Abstract from PubMed

The recently discovered cytokinin-specific phosphoribohydrolase 'Lonely Guy' (LOG) is a key enzyme of cytokinin biosynthesis, converting inactive cytokinin nucleotides into biologically active free-bases. We have determined the crystal structures of LOG from C. purpurea (cpLOG) and its complex with the enzymatic product phosphoribose. The structures reveal a dimeric arrangement of Rossmann folds, with the ligands bound to large pockets at the interface between cpLOG monomers. Structural comparisons highlight the homology of cpLOG to putative lysine decarboxylases. Extended sequence analysis enabled identification of a distinguishing LOG sequence signature. Taken together, our data suggest phosphoribohydrolase activity for several proteins of unknown function. This article is protected by copyright. All rights reserved.

The three-dimensional Structure of "Lonely Guy" from Claviceps purpurea provides insights into the phosphoribohydrolase function of Rossmann fold-containing lysine decarboxylase-like proteins.,Dzurova L, Forneris F, Savino S, Galuszka P, Vrabka J, Frebort I Proteins. 2015 May 25. doi: 10.1002/prot.24835. PMID:26010010^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Dzurova L, Forneris F, Savino S, Galuszka P, Vrabka J, Frebort I. The three-dimensional Structure of "Lonely Guy" from Claviceps purpurea provides insights into the phosphoribohydrolase function of Rossmann fold-containing lysine decarboxylase-like proteins. Proteins. 2015 May 25. doi: 10.1002/prot.24835. PMID:26010010 doi:http://dx.doi.org/10.1002/prot.24835

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5aju"

Categories: Claviceps purpurea | Large Structures | Dzurova L | Forneris F | Savino S

@@ Line 3: / Line 3: @@
 <StructureSection load='5aju' size='340' side='right'caption='[[5aju]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5aju]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clapu Clapu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AJU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AJU FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5aju]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Claviceps_purpurea Claviceps purpurea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AJU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AJU FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=RP5:5-O-PHOSPHONO-BETA-D-RIBOFURANOSE'>RP5</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5ajt|5ajt]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=RP5:5-O-PHOSPHONO-BETA-D-RIBOFURANOSE'>RP5</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5aju FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aju OCA], [http://pdbe.org/5aju PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5aju RCSB], [http://www.ebi.ac.uk/pdbsum/5aju PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5aju ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5aju FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aju OCA], [https://pdbe.org/5aju PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5aju RCSB], [https://www.ebi.ac.uk/pdbsum/5aju PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5aju ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/M1VUY5_CLAP2 M1VUY5_CLAP2]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Clapu]]
-[[Category: Large Structures]]
-[[Category: Dzurova, L]]
-[[Category: Forneris, F]]
-[[Category: Savino, S]]
 [[Category: Claviceps purpurea]]
-[[Category: Cytokinin]]
+[[Category: Large Structures]]
-[[Category: Hydrolase]]
+[[Category: Dzurova L]]
-[[Category: Lysine decarboxylase]]
+[[Category: Forneris F]]
-[[Category: Phosphoribohydrolase]]
+[[Category: Savino S]]
-[[Category: Protein lonely guy]]
-[[Category: Rossmann fold]]

5aju

From Proteopedia

Current revision

Crystal structure of ligand-free phosphoribohydroxylase lonely guy from Claviceps purpurea in complex with phosphoribose

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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