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| | ==Structure of the N-terminal domain of the metalloprotease PrtV from Vibrio cholerae== | | ==Structure of the N-terminal domain of the metalloprotease PrtV from Vibrio cholerae== |
| - | <StructureSection load='5abk' size='340' side='right'caption='[[5abk]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='5abk' size='340' side='right'caption='[[5abk]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5abk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ABK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ABK FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5abk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ABK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ABK FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5abk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5abk OCA], [http://pdbe.org/5abk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5abk RCSB], [http://www.ebi.ac.uk/pdbsum/5abk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5abk ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5abk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5abk OCA], [https://pdbe.org/5abk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5abk RCSB], [https://www.ebi.ac.uk/pdbsum/5abk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5abk ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/PRTV_VIBCH PRTV_VIBCH] Metalloprotease that exhibits a cytotoxic effect leading to cell death. In host tissues, it could play a role in pathogenesis by modulating the stability of the extracellular matrix components such as fibronectin and fibrinogen. Also able to cleave plasminogen.<ref>PMID:18479458</ref> <ref>PMID:24492010</ref> <ref>PMID:9371455</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillo virgola del koch trevisan 1884]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Edwin, A]] | |
| - | [[Category: Karlsson, G]] | |
| - | [[Category: Mayzel, M]] | |
| - | [[Category: Ohman, A]] | |
| - | [[Category: Persson, C]] | |
| - | [[Category: Sauer-Eriksson, A E]] | |
| - | [[Category: Wai, S N]] | |
| - | [[Category: Hydrolase]] | |
| - | [[Category: N-terminal domain]] | |
| - | [[Category: Prtv]] | |
| | [[Category: Vibrio cholerae]] | | [[Category: Vibrio cholerae]] |
| | + | [[Category: Edwin A]] |
| | + | [[Category: Karlsson G]] |
| | + | [[Category: Mayzel M]] |
| | + | [[Category: Ohman A]] |
| | + | [[Category: Persson C]] |
| | + | [[Category: Sauer-Eriksson AE]] |
| | + | [[Category: Wai SN]] |
| Structural highlights
Function
PRTV_VIBCH Metalloprotease that exhibits a cytotoxic effect leading to cell death. In host tissues, it could play a role in pathogenesis by modulating the stability of the extracellular matrix components such as fibronectin and fibrinogen. Also able to cleave plasminogen.[1] [2] [3]
Publication Abstract from PubMed
The metalloprotease PrtV from Vibrio cholerae serves an important function for the ability of bacteria to invade the mammalian host cell. The protein belongs to the family of M6 proteases, with a characteristic zinc ion in the catalytic active site. PrtV constitutes a 918 amino acids (102 kDa) multidomain pre-pro-protein that undergoes several N- and C- terminal modifications to form a catalytically active protease. We report here the NMR structure of the PrtV N-terminal domain (residues 23-103) that contains two short alpha-helices in a coiled coil motif. The helices are held together by a cluster of hydrophobic residues. Approximately 30 residues at the C-terminal end, which were predicted to form a third helical structure, are disordered. These residues are highly conserved within the genus Vibrio, which suggests that they might be functionally important. This article is protected by copyright. All rights reserved.
Structure of the N-terminal domain of the metalloprotease PrtV from Vibrio cholerae.,Edwin A, Persson C, Mayzel M, Wai SN, Ohman A, Karlsson BG, Sauer-Eriksson AE Protein Sci. 2015 Oct 5. doi: 10.1002/pro.2815. PMID:26434928[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Vaitkevicius K, Rompikuntal PK, Lindmark B, Vaitkevicius R, Song T, Wai SN. The metalloprotease PrtV from Vibrio cholerae. FEBS J. 2008 Jun;275(12):3167-77. PMID:18479458 doi:10.1111/j.1742-4658.2008.06470.x
- ↑ Edwin A, Grundström C, Wai SN, Ohman A, Stier G, Sauer-Eriksson AE. Domain isolation, expression, purification and proteolytic activity of the metalloprotease PrtV from Vibrio cholerae. Protein Expr Purif. 2014 Apr;96:39-47. PMID:24492010 doi:10.1016/j.pep.2014.01.012
- ↑ Ogierman MA, Fallarino A, Riess T, Williams SG, Attridge SR, Manning PA. Characterization of the Vibrio cholerae El Tor lipase operon lipAB and a protease gene downstream of the hly region. J Bacteriol. 1997 Nov;179(22):7072-80. PMID:9371455 doi:10.1128/jb.179.22.7072-7080.1997
- ↑ Edwin A, Persson C, Mayzel M, Wai SN, Ohman A, Karlsson BG, Sauer-Eriksson AE. Structure of the N-terminal domain of the metalloprotease PrtV from Vibrio cholerae. Protein Sci. 2015 Oct 5. doi: 10.1002/pro.2815. PMID:26434928 doi:http://dx.doi.org/10.1002/pro.2815
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