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| | <StructureSection load='5ave' size='340' side='right'caption='[[5ave]], [[Resolution|resolution]] 1.80Å' scene=''> | | <StructureSection load='5ave' size='340' side='right'caption='[[5ave]], [[Resolution|resolution]] 1.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5ave]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AVE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5AVE FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5ave]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AVE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AVE FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SER:SERINE'>SER</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">DN30_191 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SER:SERINE'>SER</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ave FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ave OCA], [http://pdbe.org/5ave PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ave RCSB], [http://www.ebi.ac.uk/pdbsum/5ave PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ave ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ave FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ave OCA], [https://pdbe.org/5ave PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ave RCSB], [https://www.ebi.ac.uk/pdbsum/5ave PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ave ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/A0A0H3AES3_VIBC3 A0A0H3AES3_VIBC3] |
| - | Vibrio cholerae, the etiological agent of cholera, was found to be attracted by taurine (2-aminoethanesulfonic acid), a major constituent of human bile. Mlp37, the closest homolog of the previously identified amino acid chemoreceptor Mlp24, was found to mediate taxis to taurine as well as L-serine, L-alanine, L-arginine, and other amino acids. Methylation of Mlp37 was enhanced upon the addition of taurine and amino acids. Isothermal titration calorimetry demonstrated that a purified periplasmic fragment of Mlp37 binds directly to taurine, L-serine, L-alanine and L-arginine. Crystal structures of the periplamic domain of Mlp37 revealed that L-serine and taurine bind to the membrane-distal PAS domain in essentially in the same way. The structural information was supported by characterising the in vivo properties of alanine-substituted mutant forms of Mlp37. The fact that the ligand-binding domain of the L-serine complex had a small opening, which would accommodate a larger R group, accounts for the broad ligand specificity of Mlp37 and allowed us to visualise ligand binding to Mlp37 with fluorescently labelled L-serine. Taken together, we conclude that Mlp37 serves as the major chemoreceptor for taurine and various amino acids.
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| - | Identification of a Vibrio cholerae chemoreceptor that senses taurine and amino acids as attractants.,Nishiyama S, Takahashi Y, Yamamoto K, Suzuki D, Itoh Y, Sumita K, Uchida Y, Homma M, Imada K, Kawagishi I Sci Rep. 2016 Feb 16;6:20866. doi: 10.1038/srep20866. PMID:26878914<ref>PMID:26878914</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
| + | |
| - | <div class="pdbe-citations 5ave" style="background-color:#fffaf0;"></div>
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| | | | |
| | ==See Also== | | ==See Also== |
| | *[[Chemotaxis protein 3D structures|Chemotaxis protein 3D structures]] | | *[[Chemotaxis protein 3D structures|Chemotaxis protein 3D structures]] |
| - | == References == | |
| - | <references/> | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillo virgola del koch trevisan 1884]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Imada, K]] | + | [[Category: Vibrio cholerae]] |
| - | [[Category: Kawagishi, I]] | + | [[Category: Imada K]] |
| - | [[Category: Nishiyama, S]] | + | [[Category: Kawagishi I]] |
| - | [[Category: Sumita, K]] | + | [[Category: Nishiyama S]] |
| - | [[Category: Takahashi, Y]] | + | [[Category: Sumita K]] |
| - | [[Category: Uchida, Y]] | + | [[Category: Takahashi Y]] |
| - | [[Category: Chemoreceptor]]
| + | [[Category: Uchida Y]] |
| - | [[Category: Ligand complex]]
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| - | [[Category: Mcp-like protein]]
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| - | [[Category: Pas-like domain]]
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| - | [[Category: Signaling protein]]
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