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6njy

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Current revision (14:47, 13 March 2024) (edit) (undo)
 
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<StructureSection load='6njy' size='340' side='right'caption='[[6njy]], [[Resolution|resolution]] 1.76&Aring;' scene=''>
<StructureSection load='6njy' size='340' side='right'caption='[[6njy]], [[Resolution|resolution]] 1.76&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6njy]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NJY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NJY FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6njy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mahella_australiensis_50-1_BON Mahella australiensis 50-1 BON]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NJY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NJY FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.76&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6njy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6njy OCA], [http://pdbe.org/6njy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6njy RCSB], [http://www.ebi.ac.uk/pdbsum/6njy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6njy ProSAT]</span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6njy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6njy OCA], [https://pdbe.org/6njy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6njy RCSB], [https://www.ebi.ac.uk/pdbsum/6njy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6njy ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/F3ZXK6_MAHA5 F3ZXK6_MAHA5]
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Prokaryotic CRISPR-Cas adaptive immune systems rely on small non-coding RNAs derived from CRISPR loci to recognize and destroy complementary nucleic acids. However, the mechanism of Type IV CRISPR RNA (crRNA) biogenesis is poorly understood. To dissect the mechanism of Type IV CRISPR RNA biogenesis, we determined the x-ray crystal structure of the putative Type IV CRISPR associated endoribonuclease Cas6 from Mahella australiensis (Ma Cas6-IV) and characterized its enzymatic activity with RNA cleavage assays. We show that Ma Cas6-IV specifically cleaves Type IV crRNA repeats at the 3' side of a predicted stem loop, with a metal-independent, single-turnover mechanism that relies on a histidine and a tyrosine located within the putative endonuclease active site. Structure and sequence alignments with Cas6 orthologs reveal that although Ma Cas6-IV shares little sequence homology with other Cas6 proteins, all share common structural features that bind distinct crRNA repeat sequences. This analysis of Type IV crRNA biogenesis provides a structural and biochemical framework for understanding the similarities and differences of crRNA biogenesis across multi-subunit Class 1 CRISPR immune systems.
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Structural basis of Type IV CRISPR RNA biogenesis by a Cas6 endoribonuclease.,Taylor HN, Warner EE, Armbrust MJ, Crowley VM, Olsen KJ, Jackson RN RNA Biol. 2019 Jun 28:1-10. doi: 10.1080/15476286.2019.1634965. PMID:31232162<ref>PMID:31232162</ref>
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==See Also==
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*[[Endonuclease 3D structures|Endonuclease 3D structures]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6njy" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Jackson, R N]]
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[[Category: Mahella australiensis 50-1 BON]]
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[[Category: Olsen, K J]]
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[[Category: Jackson RN]]
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[[Category: Warner, E]]
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[[Category: Olsen KJ]]
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[[Category: Ca]]
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[[Category: Warner E]]
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[[Category: Cas6]]
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[[Category: Crispr]]
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[[Category: Csf5]]
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[[Category: Nuclease]]
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[[Category: Rna]]
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[[Category: Rna binding protein]]
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Current revision

Type IV CRISPR associated RNA endonuclease Cas6 - apo form

PDB ID 6njy

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