|
|
| Line 3: |
Line 3: |
| | <StructureSection load='5ol5' size='340' side='right'caption='[[5ol5]], [[Resolution|resolution]] 2.33Å' scene=''> | | <StructureSection load='5ol5' size='340' side='right'caption='[[5ol5]], [[Resolution|resolution]] 2.33Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5ol5]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Syny3 Syny3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OL5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OL5 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5ol5]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechocystis_sp._PCC_6803_substr._Kazusa Synechocystis sp. PCC 6803 substr. Kazusa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OL5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OL5 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.329Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSX:S-OXY+CYSTEINE'>CSX</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=CSX:S-OXY+CYSTEINE'>CSX</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5ol1|5ol1]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ol5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ol5 OCA], [https://pdbe.org/5ol5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ol5 RCSB], [https://www.ebi.ac.uk/pdbsum/5ol5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ol5 ProSAT]</span></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">dnaE-N, slr0603, dnaE-C, sll1572 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1111708 SYNY3])</td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ol5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ol5 OCA], [http://pdbe.org/5ol5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ol5 RCSB], [http://www.ebi.ac.uk/pdbsum/5ol5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ol5 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/DPO3A_SYNY3 DPO3A_SYNY3] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 27: |
Line 27: |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Syny3]] | + | [[Category: Synechocystis sp. PCC 6803 substr. Kazusa]] |
| - | [[Category: Kick, L M]] | + | [[Category: Kick LM]] |
| - | [[Category: Schneider, S]] | + | [[Category: Schneider S]] |
| - | [[Category: Cyclic peptide]]
| + | |
| - | [[Category: Extein]]
| + | |
| - | [[Category: Intein]]
| + | |
| - | [[Category: Siclopp]]
| + | |
| - | [[Category: Splicing]]
| + | |
| Structural highlights
Function
DPO3A_SYNY3
Publication Abstract from PubMed
Inteins carry out protein-splicing reactions, which are utilised in protein chemistry, protein engineering and biotechnological applications. Rearrangement of the order of the domains in split-inteins results in a head-to-tail cyclisation of the target sequence, which can be used to genetically encode and express libraries of cyclic peptides. The efficiency of the splicing reaction depends on the target sequence. Here we used mass spectrometry to assess the cyclic peptide formation of different, hexameric target sequences in vivo by the DnaE split-inteins from Synechocystis sp. and Nostoc punctiforme, revealing a strong impact by the target sequence and intein on the intracellular peptide concentration. Furthermore, we determined the crystal structures of their pre-splicing complexes, which allowed us to identify F-Block Asp17 as crucial for the DnaE-mediated splicing reaction.
Mechanistic insights into cyclic peptide generation by DnaE split-inteins through quantitative and structural investigation.,Kick L, Harteis S, Koch MF, Schneider S Chembiochem. 2017 Sep 15. doi: 10.1002/cbic.201700503. PMID:28914478[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Kick L, Harteis S, Koch MF, Schneider S. Mechanistic insights into cyclic peptide generation by DnaE split-inteins through quantitative and structural investigation. Chembiochem. 2017 Sep 15. doi: 10.1002/cbic.201700503. PMID:28914478 doi:http://dx.doi.org/10.1002/cbic.201700503
|
