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| - | | + | #REDIRECT [[6kuv]] This PDB entry is obsolete and replaced by 6kuv |
| - | ==Structure of influenza D virus polymerase bound to cRNA promoter in class 2==
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| - | <StructureSection load='6ju3' size='340' side='right'caption='[[6ju3]], [[Resolution|resolution]] 4.10Å' scene=''>
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| - | == Structural highlights ==
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| - | <table><tr><td colspan='2'>[[6ju3]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Influenza_c_virus_(c/swine/oklahoma/1334/2011) Influenza c virus (c/swine/oklahoma/1334/2011)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JU3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JU3 FirstGlance]. <br>
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| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">P3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1173138 Influenza C virus (C/swine/Oklahoma/1334/2011)]), PB1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1173138 Influenza C virus (C/swine/Oklahoma/1334/2011)]), PB2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1173138 Influenza C virus (C/swine/Oklahoma/1334/2011)])</td></tr>
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| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_RNA_polymerase RNA-directed RNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.48 2.7.7.48] </span></td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ju3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ju3 OCA], [http://pdbe.org/6ju3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ju3 RCSB], [http://www.ebi.ac.uk/pdbsum/6ju3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ju3 ProSAT]</span></td></tr>
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| - | </table>
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| - | <div style="background-color:#fffaf0;">
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| - | == Publication Abstract from PubMed ==
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| - | The influenza virus polymerase uses capped RNA primers to initiate transcription, and a combination of terminal and internal de novo initiations for the two-step replication process by binding the conserved viral genomic RNA (vRNA) or complementary RNA (cRNA) promoter. Here, we determined the apo and promoter-bound influenza D polymerase structures using cryo-electron microscopy and found the polymerase has an evolutionarily conserved stable core structure with inherently flexible peripheral domains. Strikingly, two conformations (mode A and B) of the vRNA promoter were observed where the 3'-vRNA end can bind at two different sites, whereas the cRNA promoter only binds in the mode B conformation. Functional studies confirmed the critical role of the mode B conformation for vRNA synthesis via the intermediate cRNA but not for cRNA production, which is mainly regulated by the mode A conformation. Both conformations participate in the regulation of the transcription process. This work advances our understanding of the regulatory mechanisms for the synthesis of different RNA species by influenza virus polymerase and opens new opportunities for antiviral drug design.
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| - | Structural insight into RNA synthesis by influenza D polymerase.,Peng Q, Liu Y, Peng R, Wang M, Yang W, Song H, Chen Y, Liu S, Han M, Zhang X, Wang P, Yan J, Zhang B, Qi J, Deng T, Gao GF, Shi Y Nat Microbiol. 2019 Jun 17. pii: 10.1038/s41564-019-0487-5. doi:, 10.1038/s41564-019-0487-5. PMID:31209309<ref>PMID:31209309</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 6ju3" style="background-color:#fffaf0;"></div>
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| - | == References ==
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| - | <references/>
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| - | __TOC__
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| - | </StructureSection>
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| - | [[Category: Large Structures]]
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| - | [[Category: RNA-directed RNA polymerase]]
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| - | [[Category: Gao, G F]]
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| - | [[Category: Peng, Q]]
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| - | [[Category: Peng, R]]
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| - | [[Category: Qi, J]]
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| - | [[Category: Shi, Y]]
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| - | [[Category: Cryo-em]]
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| - | [[Category: Influenza d virus]]
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| - | [[Category: Polymerase]]
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| - | [[Category: Rna binding protein]]
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| - | [[Category: Viral protein]]
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| - | [[Category: Viral protein-rna complex]]
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