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4c0x

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Current revision

The crystal strucuture of PpAzoR in complex with anthraquinone-2- sulfonate

Structural highlights

4c0x is a 1 chain structure with sequence from Pseudomonas putida. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.499Å
Ligands:	, , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

AZOR1_PSEPK Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity (By similarity).

Publication Abstract from PubMed

Enzymatic degradation of azo dyes begins with the reduction of the azo bond. In this paper we report the crystal structures of the native azoreductase from Pseudomonas putida MET94 (PpAzoR) (1.60 A), of PpAzoR in complex with the anthraquinone-2-sulfonate (1.50 A) and of PpAzoR in complex with Reactive Black 5 dye (1.90 A). These structures reveal the residues and subtle changes that accompany substrate binding and release. Such changes highlight the fine control of access to the catalytic site that is required by the ping-pong mechanism, and in turn the specificity offered by the enzyme towards different substrates. The topology surrounding the active site shows novel features of substrate recognition and binding that help to explain and differentiate the substrate specificity observed among different bacterial azoreductases. This article is protected by copyright. All rights reserved.

The Crystal Structure of Pseudomonas putida AzoR: the active site revisited.,Goncalves AM, Mendes S, de Sanctis D, Martins LO, Bento I FEBS J. 2013 Oct 16. doi: 10.1111/febs.12568. PMID:24127652^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Goncalves AM, Mendes S, de Sanctis D, Martins LO, Bento I. The Crystal Structure of Pseudomonas putida AzoR: the active site revisited. FEBS J. 2013 Oct 16. doi: 10.1111/febs.12568. PMID:24127652 doi:http://dx.doi.org/10.1111/febs.12568

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4c0x"

Categories: Large Structures | Pseudomonas putida | Bento I | Goncalves AMD | De Sanctis D

@@ Line 3: / Line 3: @@
 <StructureSection load='4c0x' size='340' side='right'caption='[[4c0x]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4c0x]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_fluorescens_putidus"_flugge_1886 "bacillus fluorescens putidus" flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C0X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4C0X FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4c0x]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4C0X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4C0X FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AQN:9,10-DIOXO-9,10-DIHYDROANTHRACENE-2-SULFONIC+ACID'>AQN</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.499&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4c0x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c0x OCA], [http://pdbe.org/4c0x PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4c0x RCSB], [http://www.ebi.ac.uk/pdbsum/4c0x PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4c0x ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AQN:9,10-DIOXO-9,10-DIHYDROANTHRACENE-2-SULFONIC+ACID'>AQN</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4c0x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4c0x OCA], [https://pdbe.org/4c0x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4c0x RCSB], [https://www.ebi.ac.uk/pdbsum/4c0x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4c0x ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/AZOR1_PSEPK AZOR1_PSEPK]] Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity (By similarity).
+[https://www.uniprot.org/uniprot/AZOR1_PSEPK AZOR1_PSEPK] Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity (By similarity).
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Bacillus fluorescens putidus flugge 1886]]
 [[Category: Large Structures]]
-[[Category: Bento, I]]
+[[Category: Pseudomonas putida]]
-[[Category: Goncalves, A M.D]]
+[[Category: Bento I]]
-[[Category: Sanctis, D de]]
+[[Category: Goncalves AMD]]
-[[Category: Azoreductase]]
+[[Category: De Sanctis D]]
-[[Category: Oxidoreductase]]

4c0x

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Current revision

The crystal strucuture of PpAzoR in complex with anthraquinone-2- sulfonate

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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