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| | ==NMR SOLUTION STRUCTURE OF NT2== | | ==NMR SOLUTION STRUCTURE OF NT2== |
| - | <StructureSection load='1je9' size='340' side='right'caption='[[1je9]], [[NMR_Ensembles_of_Models | 18 NMR models]]' scene=''> | + | <StructureSection load='1je9' size='340' side='right'caption='[[1je9]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1je9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Monocled_cobra Monocled cobra]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JE9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1JE9 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1je9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Naja_kaouthia Naja kaouthia]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JE9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JE9 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1je9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1je9 OCA], [http://pdbe.org/1je9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1je9 RCSB], [http://www.ebi.ac.uk/pdbsum/1je9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1je9 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 18 models</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1je9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1je9 OCA], [https://pdbe.org/1je9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1je9 RCSB], [https://www.ebi.ac.uk/pdbsum/1je9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1je9 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/3S1C_NAJKA 3S1C_NAJKA]] Produces peripheral paralysis by blocking neuromuscular transmission at the postsynaptic site. Binds to the nicotinic acetylcholine receptor.<ref>PMID:12039691</ref> <ref>PMID:12039691</ref> | + | [https://www.uniprot.org/uniprot/3S1C_NAJKA 3S1C_NAJKA] Produces peripheral paralysis by blocking neuromuscular transmission at the postsynaptic site. Binds to the nicotinic acetylcholine receptor.<ref>PMID:12039691</ref> <ref>PMID:12039691</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | <jmolCheckbox> | | <jmolCheckbox> |
| | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/je/1je9_consurf.spt"</scriptWhenChecked> | | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/je/1je9_consurf.spt"</scriptWhenChecked> |
| - | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> | | <text>to colour the structure by Evolutionary Conservation</text> |
| | </jmolCheckbox> | | </jmolCheckbox> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Monocled cobra]] | + | [[Category: Naja kaouthia]] |
| - | [[Category: Cheng, Y]] | + | [[Category: Cheng Y]] |
| - | [[Category: Wang, J]] | + | [[Category: Wang J]] |
| - | [[Category: Wang, W]] | + | [[Category: Wang W]] |
| - | [[Category: All beta sheet protein]]
| + | |
| - | [[Category: Postsynaptic neurotoxin]]
| + | |
| - | [[Category: Toxin]]
| + | |
| - | [[Category: Veonm]]
| + | |
| Structural highlights
Function
3S1C_NAJKA Produces peripheral paralysis by blocking neuromuscular transmission at the postsynaptic site. Binds to the nicotinic acetylcholine receptor.[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Three homologous short-chain neurotoxins, named NT1, NT2 and NT3, were purified from the venom of Naja kaouthia. NT1 has an identical amino acid sequence to cobrotoxin from Naja naja atra [Biochemistry 32 (1993) 2131]. NT3 shares the same sequence with cobrotoxin b [J. Biochem. (Tokyo) 122 (1997) 1252], whereas NT2 is a novel 61-residue neurotoxin. Tests of their physiological functions indicate that NT1 shows a greater inhibition of muscle contraction induced by electrical stimulation of the nerve than do NT2 and NT3. Homonuclear proton two-dimensional NMR methods were utilized to study the solution tertiary structure of NT2. A homology model-building method was employed to predict the structure of NT3. Comparison of the structures of these three toxins shows that the surface conformation of NT1 facilitates the substituted base residues, Arg28, Arg30, and Arg36, to occupy the favorable spatial location in the central region of loop II, and the cation groups of all three arginines face out of the molecular surface of NT1. This may contribute greatly to the higher binding of NT1 with AchR compared to NT2 and NT3.
Structure-function relationship of three neurotoxins from the venom of Naja kaouthia: a comparison between the NMR-derived structure of NT2 with its homologues, NT1 and NT3.,Cheng Y, Meng Q, Wang W, Wang J Biochim Biophys Acta. 2002 Feb 11;1594(2):353-63. PMID:11904231[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Meng QX, Wang WY, Lu QM, Jin Y, Wei JF, Zhu SW, Xiong YL. A novel short neurotoxin, cobrotoxin c, from monocellate cobra (Naja kaouthia) venom: isolation and purification, primary and secondary structure determination, and tertiary structure modeling. Comp Biochem Physiol C Toxicol Pharmacol. 2002 May;132(1):113-21. PMID:12039691
- ↑ Meng QX, Wang WY, Lu QM, Jin Y, Wei JF, Zhu SW, Xiong YL. A novel short neurotoxin, cobrotoxin c, from monocellate cobra (Naja kaouthia) venom: isolation and purification, primary and secondary structure determination, and tertiary structure modeling. Comp Biochem Physiol C Toxicol Pharmacol. 2002 May;132(1):113-21. PMID:12039691
- ↑ Cheng Y, Meng Q, Wang W, Wang J. Structure-function relationship of three neurotoxins from the venom of Naja kaouthia: a comparison between the NMR-derived structure of NT2 with its homologues, NT1 and NT3. Biochim Biophys Acta. 2002 Feb 11;1594(2):353-63. PMID:11904231
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