3kyl
From Proteopedia
(Difference between revisions)
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<StructureSection load='3kyl' size='340' side='right'caption='[[3kyl]], [[Resolution|resolution]] 2.70Å' scene=''> | <StructureSection load='3kyl' size='340' side='right'caption='[[3kyl]], [[Resolution|resolution]] 2.70Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[3kyl]] is a 2 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[3kyl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Tribolium_castaneum Tribolium castaneum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KYL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3KYL FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7Å</td></tr> |
| - | <tr id=' | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3kyl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kyl OCA], [https://pdbe.org/3kyl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3kyl RCSB], [https://www.ebi.ac.uk/pdbsum/3kyl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3kyl ProSAT]</span></td></tr> |
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q0QHL8_TRICA Q0QHL8_TRICA] | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3kyl ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3kyl ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | Telomerase is a specialized DNA polymerase that extends the 3' ends of eukaryotic linear chromosomes, a process required for genomic stability and cell viability. Here we present the crystal structure of the active Tribolium castaneum telomerase catalytic subunit, TERT, bound to an RNA-DNA hairpin designed to resemble the putative RNA-templating region and telomeric DNA. The RNA-DNA hybrid adopts a helical structure, docked in the interior cavity of the TERT ring. Contacts between the RNA template and motifs 2 and B' position the solvent-accessible RNA bases close to the enzyme active site for nucleotide binding and selectivity. Nucleic acid binding induces rigid TERT conformational changes to form a tight catalytic complex. Overall, TERT-RNA template and TERT-telomeric DNA associations are remarkably similar to those observed for retroviral reverse transcriptases, suggesting common mechanistic aspects of DNA replication between the two families of enzymes. | ||
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| - | Structural basis for telomerase catalytic subunit TERT binding to RNA template and telomeric DNA.,Mitchell M, Gillis A, Futahashi M, Fujiwara H, Skordalakes E Nat Struct Mol Biol. 2010 Apr;17(4):513-8. Epub 2010 Mar 28. PMID:20357774<ref>PMID:20357774</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 3kyl" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
| - | *[[Catalytic Subunit of T. Castaneum TERT Polymerase|Catalytic Subunit of T. Castaneum TERT Polymerase]] | ||
*[[Telomerase|Telomerase]] | *[[Telomerase|Telomerase]] | ||
*[[Telomerase 3D structures|Telomerase 3D structures]] | *[[Telomerase 3D structures|Telomerase 3D structures]] | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Tribolium castaneum]] |
| - | [[Category: Skordalakes | + | [[Category: Skordalakes E]] |
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Current revision
Structure of the catalytic subunit of telomerase bound to its RNA template and telomeric DNA
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