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| | <StructureSection load='4a3y' size='340' side='right'caption='[[4a3y]], [[Resolution|resolution]] 2.15Å' scene=''> | | <StructureSection load='4a3y' size='340' side='right'caption='[[4a3y]], [[Resolution|resolution]] 2.15Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4a3y]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ophioxylon_serpentinum Ophioxylon serpentinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A3Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4A3Y FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4a3y]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rauvolfia_serpentina Rauvolfia serpentina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4A3Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4A3Y FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Raucaffricine_beta-glucosidase Raucaffricine beta-glucosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.125 3.2.1.125] </span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4a3y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a3y OCA], [http://pdbe.org/4a3y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4a3y RCSB], [http://www.ebi.ac.uk/pdbsum/4a3y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4a3y ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4a3y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4a3y OCA], [https://pdbe.org/4a3y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4a3y RCSB], [https://www.ebi.ac.uk/pdbsum/4a3y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4a3y ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/RG1_RAUSE RG1_RAUSE] Glucosidase specifically involved in alkaloid biosynthesis leading to the accumulation of several alkaloids, including ajmaline, an important plant-derived pharmaceutical used in the treatment of heart disorders.<ref>PMID:10975500</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Ophioxylon serpentinum]] | + | [[Category: Rauvolfia serpentina]] |
| - | [[Category: Raucaffricine beta-glucosidase]]
| + | [[Category: Barleben L]] |
| - | [[Category: Barleben, L]] | + | [[Category: Lin H]] |
| - | [[Category: Lin, H]] | + | [[Category: Panjikar S]] |
| - | [[Category: Panjikar, S]] | + | [[Category: Rajendran C]] |
| - | [[Category: Rajendran, C]] | + | [[Category: Ruppert M]] |
| - | [[Category: Ruppert, M]] | + | [[Category: Stoeckigt J]] |
| - | [[Category: Stoeckigt, J]] | + | [[Category: Wang M]] |
| - | [[Category: Wang, M]] | + | [[Category: Xia L]] |
| - | [[Category: Xia, L]] | + | |
| - | [[Category: Alkaloid]]
| + | |
| - | [[Category: Hydrolase]]
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| Structural highlights
Function
RG1_RAUSE Glucosidase specifically involved in alkaloid biosynthesis leading to the accumulation of several alkaloids, including ajmaline, an important plant-derived pharmaceutical used in the treatment of heart disorders.[1]
Publication Abstract from PubMed
Two similar enzymes with different biosynthetic function in one species have evolved to catalyze two distinct reactions. X-ray structures of both enzymes help reveal their most important differences. The Rauvolfia alkaloid biosynthetic network harbors two O-glucosidases: raucaffricine glucosidase (RG), which hydrolyses raucaffricine to an intermediate downstream in the ajmaline pathway, and strictosidine glucosidase (SG), which operates upstream. RG converts strictosidine, the substrate of SG, but SG does not accept raucaffricine. Now elucidation of crystal structures of RG, inactive RG-E186Q mutant, and its complexes with ligands dihydro-raucaffricine and secologanin reveals that it is the "wider gate" of RG that allows strictosidine to enter the catalytic site, whereas the "slot-like" entrance of SG prohibits access by raucaffricine. Trp392 in RG and Trp388 in SG control the gate shape and acceptance of substrates. Ser390 directs the conformation of Trp392. 3D structures, supported by site-directed mutations and kinetic data of RG and SG, provide a structural and catalytic explanation of substrate specificity and deeper insights into O-glucosidase chemistry.
Structures of Alkaloid Biosynthetic Glucosidases Decode Substrate Specificity.,Xia L, Ruppert M, Wang M, Panjikar S, Lin H, Rajendran C, Barleben L, Stockigt J ACS Chem Biol. 2011 Oct 28. PMID:22004291[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Warzecha H, Gerasimenko I, Kutchan TM, Stockigt J. Molecular cloning and functional bacterial expression of a plant glucosidase specifically involved in alkaloid biosynthesis. Phytochemistry. 2000 Aug;54(7):657-66. doi: 10.1016/s0031-9422(00)00175-8. PMID:10975500 doi:http://dx.doi.org/10.1016/s0031-9422(00)00175-8
- ↑ Xia L, Ruppert M, Wang M, Panjikar S, Lin H, Rajendran C, Barleben L, Stockigt J. Structures of Alkaloid Biosynthetic Glucosidases Decode Substrate Specificity. ACS Chem Biol. 2011 Oct 28. PMID:22004291 doi:10.1021/cb200267w
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