4gf6

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<StructureSection load='4gf6' size='340' side='right'caption='[[4gf6]], [[Resolution|resolution]] 1.10&Aring;' scene=''>
<StructureSection load='4gf6' size='340' side='right'caption='[[4gf6]], [[Resolution|resolution]] 1.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4gf6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GF6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GF6 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4gf6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GF6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GF6 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.1&#8491;</td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=0WZ:3-(1H-PYRAZOL-1-YL)-L-TYROSINE'>0WZ</scene></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0WZ:3-(1H-PYRAZOL-1-YL)-L-TYROSINE'>0WZ</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ges|4ges]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gf6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gf6 OCA], [https://pdbe.org/4gf6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gf6 RCSB], [https://www.ebi.ac.uk/pdbsum/4gf6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gf6 ProSAT]</span></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GFP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6100 AEQVI])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gf6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gf6 OCA], [http://pdbe.org/4gf6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4gf6 RCSB], [http://www.ebi.ac.uk/pdbsum/4gf6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4gf6 ProSAT]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
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[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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ET encounters jellyfish: Through the incorporation of the metal-chelating amino acid pyTyr into green fluorescent protein (GFP) from jellyfish, photoinduced electron transfer (ET) from the GFP chromophore to a bound Cu(II) ion was shown to occur within one nanosecond in a distance-dependent manner. The crystal structure of GFP with pyTyr at a specific position shows the structural basis for the nanomolar binding affinity of pyTyr to Cu(II) ions.
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Genetic incorporation of a metal-chelating amino Acid as a probe for protein electron transfer.,Liu X, Li J, Dong J, Hu C, Gong W, Wang J Angew Chem Int Ed Engl. 2012 Oct 8;51(41):10261-5. doi: 10.1002/anie.201204962., Epub 2012 Aug 31. PMID:22936654<ref>PMID:22936654</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4gf6" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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*[[Green Fluorescent Protein|Green Fluorescent Protein]]
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*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Aeqvi]]
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[[Category: Aequorea victoria]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Dong, J]]
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[[Category: Dong J]]
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[[Category: Gong, W]]
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[[Category: Gong W]]
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[[Category: Li, J]]
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[[Category: Li J]]
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[[Category: Liu, X]]
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[[Category: Liu X]]
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[[Category: Wang, J]]
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[[Category: Wang J]]
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[[Category: Beta barrel]]
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[[Category: Electron transduction]]
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[[Category: Fluorecence]]
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[[Category: Fluorescent protein]]
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Current revision

crystal structure of GFP with cuprum bound at the Incorporated metal Chelating Amino Acid PYZ151

PDB ID 4gf6

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