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| | <StructureSection load='4krr' size='340' side='right'caption='[[4krr]], [[Resolution|resolution]] 2.12Å' scene=''> | | <StructureSection load='4krr' size='340' side='right'caption='[[4krr]], [[Resolution|resolution]] 2.12Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4krr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KRR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KRR FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4krr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KRR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KRR FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.124Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CG8458, Wnt8, wntD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4krr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4krr OCA], [http://pdbe.org/4krr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4krr RCSB], [http://www.ebi.ac.uk/pdbsum/4krr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4krr ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4krr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4krr OCA], [https://pdbe.org/4krr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4krr RCSB], [https://www.ebi.ac.uk/pdbsum/4krr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4krr ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/WNT8_DROME WNT8_DROME]] Binds as a ligand to a family of frizzled seven-transmembrane receptors and acts through a cascade of genes on the nucleus. | + | [https://www.uniprot.org/uniprot/WNT8_DROME WNT8_DROME] Binds as a ligand to a family of frizzled seven-transmembrane receptors and acts through a cascade of genes on the nucleus. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Drome]] | + | [[Category: Drosophila melanogaster]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Ahn, V E]] | + | [[Category: Ahn VE]] |
| - | [[Category: Choi, H J]] | + | [[Category: Choi H-J]] |
| - | [[Category: Chu, M L.H]] | + | [[Category: Chu ML-H]] |
| - | [[Category: Daniels, D L]] | + | [[Category: Daniels DL]] |
| - | [[Category: Nusse, R]] | + | [[Category: Nusse R]] |
| - | [[Category: Weis, W I]] | + | [[Category: Weis WI]] |
| - | [[Category: Extracellular]]
| + | |
| - | [[Category: Growth factor]]
| + | |
| - | [[Category: Linker]]
| + | |
| - | [[Category: N-terminal domain]]
| + | |
| - | [[Category: Signaling protein]]
| + | |
| - | [[Category: Uncomplexed wnt]]
| + | |
| - | [[Category: Wnt]]
| + | |
| - | [[Category: Wnt signaling]]
| + | |
| - | [[Category: Wntd]]
| + | |
| Structural highlights
Function
WNT8_DROME Binds as a ligand to a family of frizzled seven-transmembrane receptors and acts through a cascade of genes on the nucleus.
Publication Abstract from PubMed
Wnts are secreted growth factors that have critical roles in cell fate determination and stem cell renewal. The Wnt/beta-catenin pathway is initiated by binding of a Wnt protein to a Frizzled (Fzd) receptor and a coreceptor, LDL receptor-related protein 5 or 6 (LRP5/6). We report the 2.1 A resolution crystal structure of a Drosophila WntD fragment encompassing the N-terminal domain and the linker that connects it to the C-terminal domain. Differences in the structures of WntD and Xenopus Wnt8, including the positions of a receptor-binding beta hairpin and a large solvent-filled cavity in the helical core, indicate conformational plasticity in the N-terminal domain that may be important for Wnt-Frizzled specificity. Structure-based mutational analysis of mouse Wnt3a shows that the linker between the N- and C-terminal domains is required for LRP6 binding. These findings provide important insights into Wnt function and evolution.
Structural Studies of Wnts and Identification of an LRP6 Binding Site.,Chu ML, Ahn VE, Choi HJ, Daniels DL, Nusse R, Weis WI Structure. 2013 Jul 2;21(7):1235-42. doi: 10.1016/j.str.2013.05.006. Epub 2013, Jun 20. PMID:23791946[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Chu ML, Ahn VE, Choi HJ, Daniels DL, Nusse R, Weis WI. Structural Studies of Wnts and Identification of an LRP6 Binding Site. Structure. 2013 Jul 2;21(7):1235-42. doi: 10.1016/j.str.2013.05.006. Epub 2013, Jun 20. PMID:23791946 doi:10.1016/j.str.2013.05.006
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