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| | <StructureSection load='1tp8' size='340' side='right'caption='[[1tp8]], [[Resolution|resolution]] 3.00Å' scene=''> | | <StructureSection load='1tp8' size='340' side='right'caption='[[1tp8]], [[Resolution|resolution]] 3.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1tp8]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Artocarpus_hirsuta Artocarpus hirsuta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TP8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TP8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1tp8]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Artocarpus_hirsutus Artocarpus hirsutus] and [https://en.wikipedia.org/wiki/Artocarpus_integer Artocarpus integer]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TP8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TP8 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=AMG:ALPHA-METHYL-D-GALACTOSIDE'>AMG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1toq|1toq]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMG:ALPHA-METHYL-D-GALACTOSIDE'>AMG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tp8 OCA], [http://pdbe.org/1tp8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1tp8 RCSB], [http://www.ebi.ac.uk/pdbsum/1tp8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1tp8 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tp8 OCA], [https://pdbe.org/1tp8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tp8 RCSB], [https://www.ebi.ac.uk/pdbsum/1tp8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tp8 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/LECA_ARTIN LECA_ARTIN] D-galactose-specific lectin, binds the T-antigen structure Gal-beta1,3-GalNAc (Thomsen-Friedenreich-antigen-specific lectin). Potent and selective stimulant of distinct T- and B-cell functions. Shows a unique ability to specifically recognize IgA-1 from human serum. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Artocarpus hirsuta]] | + | [[Category: Artocarpus hirsutus]] |
| | + | [[Category: Artocarpus integer]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Gaikwad, S M]] | + | [[Category: Gaikwad SM]] |
| - | [[Category: Katre, U V]] | + | [[Category: Katre UV]] |
| - | [[Category: Khan, M I]] | + | [[Category: Khan MI]] |
| - | [[Category: Rao, K N]] | + | [[Category: Rao KN]] |
| - | [[Category: Suresh, C G]] | + | [[Category: Suresh CG]] |
| - | [[Category: Prism i fold]]
| + | |
| - | [[Category: Carbohydrate specificity]]
| + | |
| - | [[Category: Jacalin family]]
| + | |
| - | [[Category: Methyl--d-galactose]]
| + | |
| - | [[Category: Moraceae plant lectin]]
| + | |
| - | [[Category: Post-translational modification]]
| + | |
| - | [[Category: Sugar binding protein]]
| + | |
| - | [[Category: Three-dimensional structure]]
| + | |
| Structural highlights
Function
LECA_ARTIN D-galactose-specific lectin, binds the T-antigen structure Gal-beta1,3-GalNAc (Thomsen-Friedenreich-antigen-specific lectin). Potent and selective stimulant of distinct T- and B-cell functions. Shows a unique ability to specifically recognize IgA-1 from human serum.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Based on their carbohydrate specificity, the jacalin family of lectins can be divided into two groups: galactose-specific and mannose-specific. The former are cytoplasmic proteins, whereas the latter are localized in the storage vacuoles of cells. It has been proposed that the post-translational modification in some of the lectins that splits their polypeptide chains into two may be crucial for galactose specificity. The mannose-specific members of the family are single-chain proteins that lack the above modification. Although the galactose-specific and the mannose-specific jacalin-type lectins differ in their sequences, they share a common fold: the beta-prism I fold, which is characteristic of Moraceae plant lectins. Here, two crystal structures of a jacalin-related lectin from Artocarpus hirsuta, which is specific for galactose, in complex with methyl-alpha-D-galactose are reported. The lectin crystallized in two orthorhombic forms and one hexagonal form under similar conditions. The crystals had an unusually high solvent content. The structure was solved using the molecular-replacement method using the jacalin structure as a search model. The two orthorhombic forms were refined using data to 2.5 and 3.0 A resolution, respectively. The structures of the A. hirsuta lectin and jacalin are identical. In orthorhombic form I the crystal packing provides three different micro-environments for sugar binding in the same crystal. The observed difference in the specificity for oligosaccharides between the A. hirsuta lectin and jacalin could only be explained based on differences in the molecular associations in the packing and variation of the C-terminal length of the beta-chain. The observed insecticidal activity of A. hirsuta lectin may arise from its similar fold to domain II of the unrelated delta-endotoxin from Bacillus thuringiensis.
Two orthorhombic crystal structures of a galactose-specific lectin from Artocarpus hirsuta in complex with methyl-alpha-D-galactose.,Rao KN, Suresh CG, Katre UV, Gaikwad SM, Khan MI Acta Crystallogr D Biol Crystallogr. 2004 Aug;60(Pt 8):1404-12. Epub 2004, Jul 21. PMID:15272163[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Rao KN, Suresh CG, Katre UV, Gaikwad SM, Khan MI. Two orthorhombic crystal structures of a galactose-specific lectin from Artocarpus hirsuta in complex with methyl-alpha-D-galactose. Acta Crystallogr D Biol Crystallogr. 2004 Aug;60(Pt 8):1404-12. Epub 2004, Jul 21. PMID:15272163 doi:10.1107/S090744490401354X
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