6sa1
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6sa1 is ON HOLD Authors: Brissett, N.C., Doherty, A.J. Description: Post catalytic complex of Prim-PolC from Mycobacterium smegmatis with gapped DN...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Post catalytic complex of Prim-PolC from Mycobacterium smegmatis with gapped DNA and 3'-dUTP== | |
| + | <StructureSection load='6sa1' size='340' side='right'caption='[[6sa1]], [[Resolution|resolution]] 2.01Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6sa1]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SA1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6SA1 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.01Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=L2B:[(2~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-4-oxidanyl-oxolan-2-yl]methyl+dihydrogen+phosphate'>L2B</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PPV:PYROPHOSPHATE'>PPV</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=U3H:3-DEOXY-URIDINE+5-TRIPHOSPHATE'>U3H</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6sa1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sa1 OCA], [https://pdbe.org/6sa1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6sa1 RCSB], [https://www.ebi.ac.uk/pdbsum/6sa1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6sa1 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0R5T1_MYCS2 A0R5T1_MYCS2] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Cells utilise specialized polymerases from the Primase-Polymerase (Prim-Pol) superfamily to maintain genome stability. Prim-Pol's function in genome maintenance pathways including replication, repair and damage tolerance. Mycobacteria contain multiple Prim-Pols required for lesion repair, including Prim-PolC that performs short gap repair synthesis during excision repair. To understand the molecular basis of Prim-PolC's gap recognition and synthesis activities, we elucidated crystal structures of pre- and post-catalytic complexes bound to gapped DNA substrates. These intermediates explain its binding preference for short gaps and reveal a distinctive modus operandi called Synthesis-dependent Template Displacement (STD). This mechanism enables Prim-PolC to couple primer extension with template base dislocation, ensuring that the unpaired templating bases in the gap are ushered into the active site in an ordered manner. Insights provided by these structures establishes the molecular basis of Prim-PolC's gap recognition and extension activities, while also illuminating the mechanisms of primer extension utilised by closely related Prim-Pols. | ||
| - | + | Molecular basis for DNA repair synthesis on short gaps by mycobacterial Primase-Polymerase C.,Brissett NC, Zabrady K, Plocinski P, Bianchi J, Korycka-Machala M, Brzostek A, Dziadek J, Doherty AJ Nat Commun. 2020 Aug 21;11(1):4196. doi: 10.1038/s41467-020-18012-8. PMID:32826907<ref>PMID:32826907</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: Brissett | + | <div class="pdbe-citations 6sa1" style="background-color:#fffaf0;"></div> |
| - | [[Category: Doherty | + | == References == |
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Mycolicibacterium smegmatis MC2 155]] | ||
| + | [[Category: Synthetic construct]] | ||
| + | [[Category: Brissett NC]] | ||
| + | [[Category: Doherty AJ]] | ||
Current revision
Post catalytic complex of Prim-PolC from Mycobacterium smegmatis with gapped DNA and 3'-dUTP
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