6rtc
From Proteopedia
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==Structure of murine Solute Carrier 26 family member A9 (Slc26a9) anion transporter in the inward-facing state== | ==Structure of murine Solute Carrier 26 family member A9 (Slc26a9) anion transporter in the inward-facing state== | ||
| - | < | + | <SX load='6rtc' size='340' side='right' viewer='molstar' caption='[[6rtc]], [[Resolution|resolution]] 3.96Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6rtc]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RTC OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[6rtc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RTC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6RTC FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.96Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6rtc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rtc OCA], [https://pdbe.org/6rtc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6rtc RCSB], [https://www.ebi.ac.uk/pdbsum/6rtc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6rtc ProSAT]</span></td></tr> |
</table> | </table> | ||
== Function == | == Function == | ||
| - | [ | + | [https://www.uniprot.org/uniprot/S26A9_MOUSE S26A9_MOUSE] Ion transporter that can act both as an ion channel and anion exchanger (PubMed:15800055, PubMed:17120765, PubMed:31339488). Mainly acts as a chloride channel, which mediate uncoupled chloride anion transport in an alternate-access mechanism where a saturable binding site is alternately exposed to either one or the other side of the membrane (PubMed:31339488). Also acts as a DIDS- and thiosulfate- sensitive anion exchanger the exchange of chloride for bicarbonate ions across the cell membrane (PubMed:15800055).<ref>PMID:15800055</ref> <ref>PMID:17120765</ref> <ref>PMID:31339488</ref> |
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The epithelial anion transporter SLC26A9 contributes to airway surface hydration and gastric acid production. Colocalizing with CFTR, SLC26A9 has been proposed as a target for the treatment of cystic fibrosis. To provide molecular details of its transport mechanism, we present cryo-EM structures and a functional characterization of murine Slc26a9. These structures define the general architecture of eukaryotic SLC26 family members and reveal an unusual mode of oligomerization which relies predominantly on the cytosolic STAS domain. Our data illustrates conformational transitions of Slc26a9, supporting a rapid alternate-access mechanism which mediates uncoupled chloride transport with negligible bicarbonate or sulfate permeability. The characterization of structure-guided mutants illuminates the properties of the ion transport path, including a selective anion binding site located in the center of a mobile module within the transmembrane domain. This study thus provides a structural foundation for the understanding of the entire SLC26 family and potentially facilitates their therapeutic exploitation. | ||
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| + | Cryo-EM structures and functional characterization of murine Slc26a9 reveal mechanism of uncoupled chloride transport.,Walter JD, Sawicka M, Dutzler R Elife. 2019 Jul 24;8. pii: 46986. doi: 10.7554/eLife.46986. PMID:31339488<ref>PMID:31339488</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6rtc" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
| - | </ | + | </SX> |
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Mus musculus]] |
| - | [[Category: | + | [[Category: Dutzler R]] |
| - | [[Category: | + | [[Category: Sawicka M]] |
| - | [[Category: | + | [[Category: Walter JD]] |
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Current revision
Structure of murine Solute Carrier 26 family member A9 (Slc26a9) anion transporter in the inward-facing state
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