2vdd

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[[Image:2vdd.jpg|left|200px]]
 
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==Crystal Structure of the Open State of TolC Outer Membrane Component of Mutlidrug Efflux Pumps==
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The line below this paragraph, containing "STRUCTURE_2vdd", creates the "Structure Box" on the page.
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<StructureSection load='2vdd' size='340' side='right'caption='[[2vdd]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2vdd]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VDD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VDD FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
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{{STRUCTURE_2vdd| PDB=2vdd | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vdd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vdd OCA], [https://pdbe.org/2vdd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vdd RCSB], [https://www.ebi.ac.uk/pdbsum/2vdd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vdd ProSAT]</span></td></tr>
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</table>
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'''CRYSTAL STRUCTURE OF THE OPEN STATE OF TOLC OUTER MEMBRANE COMPONENT OF MUTLIDRUG EFFLUX PUMPS'''
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== Function ==
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[https://www.uniprot.org/uniprot/TOLC_ECOLI TOLC_ECOLI] Outer membrane channel, which is required for the function of several efflux systems such as AcrAB-TolC, AcrEF-TolC, EmrAB-TolC and MacAB-TolC. These systems are involved in export of antibiotics and other toxic compounds from the cell. TolC is also involved in import of colicin E1 into the cells.<ref>PMID:6337123</ref> <ref>PMID:11274125</ref> <ref>PMID:15228545</ref> <ref>PMID:18955484</ref> <ref>PMID:23176499</ref>
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vd/2vdd_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vdd ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
Drugs and certain proteins are transported across the membranes of Gram-negative bacteria by energy-activated pumps. The outer membrane component of these pumps is a channel that opens from a sealed resting state during the transport process. We describe two crystal structures of the Escherichia coli outer membrane protein TolC in its partially open state. Opening is accompanied by the exposure of three shallow intraprotomer grooves in the TolC trimer, where our mutagenesis data identify a contact point with the periplasmic component of a drug efflux pump, AcrA. We suggest that the assembly of multidrug efflux pumps is accompanied by induced fit of TolC driven mainly by accommodation of the periplasmic component.
Drugs and certain proteins are transported across the membranes of Gram-negative bacteria by energy-activated pumps. The outer membrane component of these pumps is a channel that opens from a sealed resting state during the transport process. We describe two crystal structures of the Escherichia coli outer membrane protein TolC in its partially open state. Opening is accompanied by the exposure of three shallow intraprotomer grooves in the TolC trimer, where our mutagenesis data identify a contact point with the periplasmic component of a drug efflux pump, AcrA. We suggest that the assembly of multidrug efflux pumps is accompanied by induced fit of TolC driven mainly by accommodation of the periplasmic component.
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==About this Structure==
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Assembly and channel opening in a bacterial drug efflux machine.,Bavro VN, Pietras Z, Furnham N, Perez-Cano L, Fernandez-Recio J, Pei XY, Misra R, Luisi B Mol Cell. 2008 Apr 11;30(1):114-21. PMID:18406332<ref>PMID:18406332</ref>
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2VDD is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VDD OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Assembly and channel opening in a bacterial drug efflux machine., Bavro VN, Pietras Z, Furnham N, Perez-Cano L, Fernandez-Recio J, Pei XY, Misra R, Luisi B, Mol Cell. 2008 Apr 11;30(1):114-21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18406332 18406332]
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</div>
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[[Category: Escherichia coli]]
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<div class="pdbe-citations 2vdd" style="background-color:#fffaf0;"></div>
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[[Category: Single protein]]
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== References ==
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[[Category: Bavro, V N.]]
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<references/>
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[[Category: Fernandez-Recio, J.]]
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__TOC__
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[[Category: Furnham, N.]]
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</StructureSection>
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[[Category: Luisi, B.]]
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[[Category: Escherichia coli K-12]]
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[[Category: Misra, R.]]
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[[Category: Large Structures]]
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[[Category: Pei, X Y.]]
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[[Category: Bavro VN]]
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[[Category: Perez-Cano, L.]]
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[[Category: Fernandez-Recio J]]
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[[Category: Pietras, Z.]]
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[[Category: Furnham N]]
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[[Category: Truer, R.]]
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[[Category: Luisi B]]
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[[Category: Alpha helical barrel]]
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[[Category: Misra R]]
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[[Category: Beta barrel]]
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[[Category: Pei XY]]
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[[Category: Integral membrane protein]]
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[[Category: Perez-Cano L]]
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[[Category: Membrane]]
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[[Category: Pietras Z]]
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[[Category: Multidrug efflux pump]]
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[[Category: Truer R]]
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[[Category: Outer membrane]]
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[[Category: Transmembrane]]
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[[Category: Transport]]
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[[Category: Transport protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr 24 09:42:50 2008''
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Current revision

Crystal Structure of the Open State of TolC Outer Membrane Component of Mutlidrug Efflux Pumps

PDB ID 2vdd

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