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2v17

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Structure of the complex of antibody MN423 with a fragment of tau protein

Structural highlights

2v17 is a 3 chain structure with sequence from Homo sapiens and Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.65Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The major constituent of Alzheimer's disease paired helical filaments (PHF) core is intrinsically disordered protein (IDP) tau. In spite of a considerable effort, insoluble character of PHF together with inherent physical properties of IDP tau have precluded so far reconstruction of PHF 3D structure by X-ray crystallography or NMR spectroscopy. Here we present first crystallographic study of PHF core C-terminus. Using monoclonal antibody MN423 specific to the tertiary structure of the PHF core, the in vivo PHF structure was imprinted into recombinant core PHF tau. Crystallization of the complex led to determination of the structure of the core PHF tau protein fragment 386TDHGAE391 at 1.65A resolution. Structural analysis suggests important role of the core PHF C-terminus for PHF assembly. It is reasonable to expect that this approach will help to reveal the structural principles underlying the tau protein assembly into PHF and possibly will facilitate rationale drug design for inhibition of Alzheimer neurofibrillary changes.

X-ray structure of the PHF core C-terminus: insight into the folding of the intrinsically disordered protein tau in Alzheimer's disease.,Sevcik J, Skrabana R, Dvorsky R, Csokova N, Iqbal K, Novak M FEBS Lett. 2007 Dec 22;581(30):5872-8. Epub 2007 Dec 3. PMID:18061582^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Sevcik J, Skrabana R, Dvorsky R, Csokova N, Iqbal K, Novak M. X-ray structure of the PHF core C-terminus: insight into the folding of the intrinsically disordered protein tau in Alzheimer's disease. FEBS Lett. 2007 Dec 22;581(30):5872-8. Epub 2007 Dec 3. PMID:18061582 doi:10.1016/j.febslet.2007.11.067

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2v17"

@@ Line 3: / Line 3: @@
 <StructureSection load='2v17' size='340' side='right'caption='[[2v17]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2v17]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V17 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2V17 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2v17]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2V17 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2V17 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v17 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v17 OCA], [http://pdbe.org/2v17 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2v17 RCSB], [http://www.ebi.ac.uk/pdbsum/2v17 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2v17 ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2v17 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v17 OCA], [https://pdbe.org/2v17 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2v17 RCSB], [https://www.ebi.ac.uk/pdbsum/2v17 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2v17 ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 28: / Line 29: @@
 ==See Also==
 *[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
-*[[Structure and dynamics of the microtubule-binding hot-spots on the neuronal protein tau|Structure and dynamics of the microtubule-binding hot-spots on the neuronal protein tau]]
+*[[Tau protein 3D structures|Tau protein 3D structures]]
-*[[Tau protein|Tau protein]]
 == References ==
 <references/>
@@ Line 37: / Line 37: @@
 [[Category: Large Structures]]
 [[Category: Mus musculus]]
-[[Category: Csokova, N]]
+[[Category: Csokova N]]
-[[Category: Dvorsky, R]]
+[[Category: Dvorsky R]]
-[[Category: Novak, M]]
+[[Category: Novak M]]
-[[Category: Sevcik, J]]
+[[Category: Sevcik J]]
-[[Category: Skrabana, R]]
+[[Category: Skrabana R]]
-[[Category: Alzheimer's disease]]
-[[Category: Immune system]]
-[[Category: Microtubule]]
-[[Category: Tau protein]]

2v17

From Proteopedia

Current revision

Structure of the complex of antibody MN423 with a fragment of tau protein

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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