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| | <StructureSection load='6j36' size='340' side='right'caption='[[6j36]], [[Resolution|resolution]] 2.30Å' scene=''> | | <StructureSection load='6j36' size='340' side='right'caption='[[6j36]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6j36]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"mycoplasma_suipneumoniae"_goodwin_et_al._1965 "mycoplasma suipneumoniae" goodwin et al. 1965]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J36 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6J36 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6j36]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mesomycoplasma_hyopneumoniae Mesomycoplasma hyopneumoniae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6J36 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6J36 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.301Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">eno ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2099 "Mycoplasma suipneumoniae" Goodwin et al. 1965])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphopyruvate_hydratase Phosphopyruvate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.11 4.2.1.11] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6j36 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j36 OCA], [https://pdbe.org/6j36 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6j36 RCSB], [https://www.ebi.ac.uk/pdbsum/6j36 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6j36 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6j36 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6j36 OCA], [http://pdbe.org/6j36 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6j36 RCSB], [http://www.ebi.ac.uk/pdbsum/6j36 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6j36 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/A0A223MA21_MYCHY A0A223MA21_MYCHY]] Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.[HAMAP-Rule:MF_00318] | + | [https://www.uniprot.org/uniprot/A0A223MA21_MESHO A0A223MA21_MESHO] Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.[HAMAP-Rule:MF_00318] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Mycoplasma suipneumoniae goodwin et al. 1965]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Phosphopyruvate hydratase]] | + | [[Category: Mesomycoplasma hyopneumoniae]] |
| - | [[Category: Chen, R]] | + | [[Category: Chen R]] |
| - | [[Category: Feng, Z]] | + | [[Category: Feng Z]] |
| - | [[Category: Gan, R]] | + | [[Category: Gan R]] |
| - | [[Category: Ran, T]] | + | [[Category: Ran T]] |
| - | [[Category: Shao, G]] | + | [[Category: Shao G]] |
| - | [[Category: Wang, W]] | + | [[Category: Wang W]] |
| - | [[Category: Xiang, Q]] | + | [[Category: Xiang Q]] |
| - | [[Category: Xie, X]] | + | [[Category: Xie X]] |
| - | [[Category: Zhang, S]] | + | [[Category: Zhang S]] |
| - | [[Category: Zhang, W]] | + | [[Category: Zhang W]] |
| - | [[Category: Cell adhesion]]
| + | |
| - | [[Category: Octamer]]
| + | |
| Structural highlights
Function
A0A223MA21_MESHO Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.[HAMAP-Rule:MF_00318]
Publication Abstract from PubMed
Enolase is an evolutionarily conserved enzyme involved in the processes of glycolysis and gluconeogenesis. Mycoplasma hyopneumoniae belongs to Mycoplasma, whose species are wall-less and among the smallest self-replicating bacteria, and is an important colonizing respiratory pathogen in the pig industry worldwide. Mycoplasma hyopneumoniae enolase (Mhp Eno) expression is significantly increased after infection and was previously found to be a virulence factor candidate. Our studies show that Mhp Eno is a cell surface-localized protein that can adhere to swine tracheal epithelial cells (STECs). Adhesion to STECs can be specifically inhibited by an Mhp Eno antibody. Mhp Eno can recognize and interact with plasminogen with high affinity. Here, the first crystal structure of the mycoplasmal enolase from Mycoplasma hyopneumoniae was determined. The structure showed unique features of Mhp Eno in the S3/H1, H6/S6, H7/H8, and H13 regions. All of these regions were longer than those of other enolases and were exposed on the Mhp Eno surface, making them accessible to host molecules. These results show that Mhp Eno has specific structural characteristics and acts as a multifunctional adhesin on the Mycoplasma hyopneumoniae cell surface.
Featured Species-Specific Loops Are Found in the Crystal Structure of Mhp Eno, a Cell Surface Adhesin From Mycoplasma hyopneumoniae.,Chen R, Yu Y, Feng Z, Gan R, Xie X, Zhang Z, Xie Q, Wang W, Ran T, Zhang W, Xiong Q, Shao G Front Cell Infect Microbiol. 2019 Jun 13;9:209. doi: 10.3389/fcimb.2019.00209., eCollection 2019. PMID:31263685[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Chen R, Yu Y, Feng Z, Gan R, Xie X, Zhang Z, Xie Q, Wang W, Ran T, Zhang W, Xiong Q, Shao G. Featured Species-Specific Loops Are Found in the Crystal Structure of Mhp Eno, a Cell Surface Adhesin From Mycoplasma hyopneumoniae. Front Cell Infect Microbiol. 2019 Jun 13;9:209. doi: 10.3389/fcimb.2019.00209., eCollection 2019. PMID:31263685 doi:http://dx.doi.org/10.3389/fcimb.2019.00209
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