2je3

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[[Image:2je3.gif|left|200px]]<br />
 
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<applet load="2je3" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2je3, resolution 1.80&Aring;" />
 
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'''CYTOCHROME P460 FROM NITROSOMONAS EUROPAEA- PROBABLE PHYSIOLOGICAL FORM'''<br />
 
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==Overview==
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==Cytochrome P460 from Nitrosomonas europaea - probable physiological form==
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We have determined the 1.8 A X-ray crystal structure of a monoheme c-type, cytochrome, cytochrome P460, from Nitrosomonas europea. The chromophore, possesses unusual spectral properties analogous to those of the catalytic, heme P460 of hydroxylamine oxidoreductase (HAO), the only known heme in, biology to withdraw electrons from an iron-coordinated substrate. The, analysis reveals a homodimeric structure and elucidates a new c-type, cytochrome fold that is predominantly beta-sheet. In addition to the two, cysteine thioether links to the porphyrin typical of c-type hemes, there, is a third proteinaceous link involving a conserved lysine. The covalent, bond is between the lysine side-chain nitrogen and the 13'-meso carbon of, the heme, which, following cross-link formation, is sp3-hybridized, demonstrating the loss of conjugation at this position within the, porphyrin. The structure has implications for the analogous tyrosine-heme, meso carbon cross-link observed in HAO.
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<StructureSection load='2je3' size='340' side='right'caption='[[2je3]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2je3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nitrosomonas_europaea Nitrosomonas europaea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JE3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JE3 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2je3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2je3 OCA], [https://pdbe.org/2je3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2je3 RCSB], [https://www.ebi.ac.uk/pdbsum/2je3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2je3 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q50927_NITER Q50927_NITER]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/je/2je3_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2je3 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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We have determined the 1.8 A X-ray crystal structure of a monoheme c-type cytochrome, cytochrome P460, from Nitrosomonas europea. The chromophore possesses unusual spectral properties analogous to those of the catalytic heme P460 of hydroxylamine oxidoreductase (HAO), the only known heme in biology to withdraw electrons from an iron-coordinated substrate. The analysis reveals a homodimeric structure and elucidates a new c-type cytochrome fold that is predominantly beta-sheet. In addition to the two cysteine thioether links to the porphyrin typical of c-type hemes, there is a third proteinaceous link involving a conserved lysine. The covalent bond is between the lysine side-chain nitrogen and the 13'-meso carbon of the heme, which, following cross-link formation, is sp3-hybridized, demonstrating the loss of conjugation at this position within the porphyrin. The structure has implications for the analogous tyrosine-heme meso carbon cross-link observed in HAO.
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==About this Structure==
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The crystal structure of cytochrome P460 of Nitrosomonas europaea reveals a novel cytochrome fold and heme-protein cross-link.,Pearson AR, Elmore BO, Yang C, Ferrara JD, Hooper AB, Wilmot CM Biochemistry. 2007 Jul 17;46(28):8340-9. Epub 2007 Jun 21. PMID:17583915<ref>PMID:17583915</ref>
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2JE3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Nitrosomonas_europaea Nitrosomonas europaea] with PO4 and HEC as [http://en.wikipedia.org/wiki/ligands ligands]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2JE3 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The Crystal Structure of Cytochrome P460 of Nitrosomonas europaea Reveals a Novel Cytochrome Fold and Heme-Protein Cross-link(,)., Pearson AR, Elmore BO, Yang C, Ferrara JD, Hooper AB, Wilmot CM, Biochemistry. 2007 Jul 17;46(28):8340-9. Epub 2007 Jun 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17583915 17583915]
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</div>
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<div class="pdbe-citations 2je3" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Nitrosomonas europaea]]
[[Category: Nitrosomonas europaea]]
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[[Category: Single protein]]
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[[Category: Elmore BO]]
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[[Category: Elmore, B.O.]]
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[[Category: Ferrara JD]]
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[[Category: Ferrara, J.D.]]
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[[Category: Hooper AB]]
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[[Category: Hooper, A.B.]]
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[[Category: Pearson AR]]
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[[Category: Pearson, A.R.]]
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[[Category: Wilmot CM]]
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[[Category: Wilmot, C.M.]]
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[[Category: Yang C]]
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[[Category: Yang, C.]]
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[[Category: HEC]]
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[[Category: PO4]]
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[[Category: cross-linked heme]]
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[[Category: cytochrome p460]]
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[[Category: heme p460]]
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[[Category: metal binding protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 18:32:27 2007''
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Current revision

Cytochrome P460 from Nitrosomonas europaea - probable physiological form

PDB ID 2je3

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