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6scr
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(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6scr is ON HOLD Authors: Maveyraud, L., Garcia-Alles, L.F., Mourey, L. Description: Structure of CcmK4 from Synechocystis sp. PCC6803 [[Category: U...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of CcmK4 from Synechocystis sp. PCC6803== | |
| + | <StructureSection load='6scr' size='340' side='right'caption='[[6scr]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6scr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechocystis_sp._PCC_6803_substr._Kazusa Synechocystis sp. PCC 6803 substr. Kazusa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SCR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6SCR FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6scr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6scr OCA], [https://pdbe.org/6scr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6scr RCSB], [https://www.ebi.ac.uk/pdbsum/6scr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6scr ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/CCMK4_SYNY3 CCMK4_SYNY3] May be involved in the formation of the carboxysome, a polyhedral inclusion where RuBisCO is sequestered (By similarity). | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The carboxysome is a bacterial micro-compartment (BMC) subtype that encapsulates enzymatic activities necessary for carbon fixation. Carboxysome shells are composed of a relatively complex cocktail of proteins, their precise number and identity being species dependent. Shell components can be classified in two structural families, the most abundant class associating as hexamers (BMC-H) that are supposed to be major players for regulating shell permeability. Up to recently, these proteins were proposed to associate as homo-oligomers. Genomic data, however, demonstrated the existence of paralogs coding for multiple shell subunits. Here, we studied cross-association compatibilities among BMC-H CcmK proteins of Synechocystis sp. PCC6803. Co-expression in Escherichia coli proved a consistent formation of hetero-hexamers combining CcmK1 and CcmK2 or, remarkably, CcmK3 and CcmK4 subunits. Unlike CcmK1/K2 hetero-hexamers, the stoichiometry of incorporation of CcmK3 in associations with CcmK4 was low. Cross-interactions implicating other combinations were weak, highlighting a structural segregation of the two groups that could relate to gene organization. Sequence analysis and structural models permitted the localization of interactions that would favor formation of CcmK3/K4 hetero-hexamers. The crystallization of these CcmK3/K4 associations conducted to the elucidation of a structure corresponding to the CcmK4 homo-hexamer. Yet, subunit exchange could not be demonstrated in vitro. Biophysical measurements showed that hetero-hexamers are thermally less stable than homo-hexamers, and impeded in forming larger assemblies. These novel findings are discussed within the context of reported data to propose a functional scenario in which minor CcmK3/K4 incorporation in shells would introduce sufficient local disorder as to allow shell remodeling necessary to adapt rapidly to environmental changes. | ||
| - | + | Occurrence and stability of hetero-hexamer associations formed by beta-carboxysome CcmK shell components.,Garcia-Alles LF, Root K, Maveyraud L, Aubry N, Lesniewska E, Mourey L, Zenobi R, Truan G PLoS One. 2019 Oct 11;14(10):e0223877. doi: 10.1371/journal.pone.0223877., eCollection 2019. PMID:31603944<ref>PMID:31603944</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 6scr" style="background-color:#fffaf0;"></div> |
| - | [[Category: Garcia-Alles | + | == References == |
| - | [[Category: Maveyraud | + | <references/> |
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Synechocystis sp. PCC 6803 substr. Kazusa]] | ||
| + | [[Category: Garcia-Alles LF]] | ||
| + | [[Category: Maveyraud L]] | ||
| + | [[Category: Mourey L]] | ||
Current revision
Structure of CcmK4 from Synechocystis sp. PCC6803
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