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| | <StructureSection load='3cr8' size='340' side='right'caption='[[3cr8]], [[Resolution|resolution]] 2.95Å' scene=''> | | <StructureSection load='3cr8' size='340' side='right'caption='[[3cr8]], [[Resolution|resolution]] 2.95Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3cr8]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"thiobacillus_denitrificans"_beijerinck_1904 "thiobacillus denitrificans" beijerinck 1904]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CR8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3CR8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3cr8]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Thiobacillus_denitrificans Thiobacillus denitrificans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CR8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CR8 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3cr8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cr8 OCA], [http://pdbe.org/3cr8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3cr8 RCSB], [http://www.ebi.ac.uk/pdbsum/3cr8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3cr8 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.95Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cr8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cr8 OCA], [https://pdbe.org/3cr8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cr8 RCSB], [https://www.ebi.ac.uk/pdbsum/3cr8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cr8 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q3SM86_THIDA Q3SM86_THIDA] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Thiobacillus denitrificans beijerinck 1904]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Fisher, A J]] | + | [[Category: Thiobacillus denitrificans]] |
| - | [[Category: Gay, S C]] | + | [[Category: Fisher AJ]] |
| - | [[Category: Segel, I H]] | + | [[Category: Gay SC]] |
| - | [[Category: Adenylylsulfate kinase]] | + | [[Category: Segel IH]] |
| - | [[Category: Aps kinase]]
| + | |
| - | [[Category: Nucleotide 2 kinase]]
| + | |
| - | [[Category: Nucleotidyltransferase]]
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| - | [[Category: Sulfate metabolism]]
| + | |
| - | [[Category: Transferase]]
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| Structural highlights
Function
Q3SM86_THIDA
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The Tbd_0210 gene of the chemolithotrophic bacterium Thiobacillus denitrificans is annotated to encode a 60.5 kDa bifunctional enzyme with ATP sulfurylase and APS kinase activity. This putative bifunctional enzyme was cloned, expressed and structurally characterized. The 2.95 A resolution X-ray crystal structure reported here revealed a hexameric assembly with D(3) symmetry. Each subunit contains a large N-terminal sulfurylase-like domain and a C-terminal APS kinase domain reminiscent of the two-domain fungal ATP sulfurylases of Penicillium chrysogenum and Saccharomyces cerevisiae, which also exhibit a hexameric assembly. However, the T. denitrificans enzyme exhibits numerous structural and sequence differences in the N-terminal domain that render it inactive with respect to ATP sulfurylase activity. Surprisingly, the C-terminal domain does indeed display APS kinase activity, indicating that this gene product is a true APS kinase. Therefore, these results provide the first structural insights into a unique hexameric APS kinase that contains a nonfunctional ATP sulfurylase-like domain of unknown function.
Structure of the two-domain hexameric APS kinase from Thiobacillus denitrificans: structural basis for the absence of ATP sulfurylase activity.,Gay SC, Segel IH, Fisher AJ Acta Crystallogr D Biol Crystallogr. 2009 Oct;65(Pt 10):1021-31. Epub 2009, Sep 16. PMID:19770499[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Gay SC, Segel IH, Fisher AJ. Structure of the two-domain hexameric APS kinase from Thiobacillus denitrificans: structural basis for the absence of ATP sulfurylase activity. Acta Crystallogr D Biol Crystallogr. 2009 Oct;65(Pt 10):1021-31. Epub 2009, Sep 16. PMID:19770499 doi:10.1107/S0907444909026547
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