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| | <StructureSection load='3e1h' size='340' side='right'caption='[[3e1h]], [[Resolution|resolution]] 2.58Å' scene=''> | | <StructureSection load='3e1h' size='340' side='right'caption='[[3e1h]], [[Resolution|resolution]] 2.58Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3e1h]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chrysonilia_crassa Chrysonilia crassa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E1H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3E1H FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3e1h]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Neurospora_crassa Neurospora crassa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E1H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3E1H FirstGlance]. <br> |
| - | </td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Naringenin-chalcone_synthase Naringenin-chalcone synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.74 2.3.1.74] </span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.58Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3e1h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e1h OCA], [http://pdbe.org/3e1h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3e1h RCSB], [http://www.ebi.ac.uk/pdbsum/3e1h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3e1h ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3e1h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e1h OCA], [https://pdbe.org/3e1h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3e1h RCSB], [https://www.ebi.ac.uk/pdbsum/3e1h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3e1h ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q7S6N4_NEUCR Q7S6N4_NEUCR] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Chrysonilia crassa]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Naringenin-chalcone synthase]] | + | [[Category: Neurospora crassa]] |
| - | [[Category: Goyal, A]] | + | [[Category: Goyal A]] |
| - | [[Category: Rahman, A]] | + | [[Category: Rahman A]] |
| - | [[Category: Sankaranarayanan, R]] | + | [[Category: Sankaranarayanan R]] |
| - | [[Category: Acyltransferase]]
| + | |
| - | [[Category: Resorcinolic lipid synthase]]
| + | |
| - | [[Category: Transferase]]
| + | |
| - | [[Category: Type iii pk]]
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| Structural highlights
Function
Q7S6N4_NEUCR
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Microbial type III polyketide synthases (PKSs) have revealed remarkable mechanistic as well as functional versatility. Recently, a type III PKS homolog from Azotobacter has been implicated in the biosynthesis of resorcinolic lipids, thus adding a new functional significance to this class of proteins. Here, we report the structural and mutational investigations of a novel type III PKS protein from Neurospora crassa involved in the biosynthesis of resorcinolic metabolites by utilizing long chain fatty acyl-CoAs. The structure revealed a long hydrophobic tunnel responsible for its fatty acyl chain length specificity resembling that of PKS18, a mycobacterial type III PKS. Structure-based mutational studies to block the tunnel not only altered the fatty acyl chain specificity but also resulted in change of cyclization pattern affecting the product profile. This first structural characterization of a resorcinolic lipid synthase provides insights into the coordinated functioning of cyclization and a substrate-binding pocket, which shows mechanistic intricacy underlying type III PKS catalysis.
Structural insights into biosynthesis of resorcinolic lipids by a type III polyketide synthase in Neurospora crassa.,Goyal A, Saxena P, Rahman A, Singh PK, Kasbekar DP, Gokhale RS, Sankaranarayanan R J Struct Biol. 2008 Jun;162(3):411-21. Epub 2008 Mar 30. PMID:18462950[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Goyal A, Saxena P, Rahman A, Singh PK, Kasbekar DP, Gokhale RS, Sankaranarayanan R. Structural insights into biosynthesis of resorcinolic lipids by a type III polyketide synthase in Neurospora crassa. J Struct Biol. 2008 Jun;162(3):411-21. Epub 2008 Mar 30. PMID:18462950 doi:10.1016/j.jsb.2008.02.009
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