This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

1nh9

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Current revision

Crystal Structure of a DNA Binding Protein Mja10b from the hyperthermophile Methanococcus jannaschii

Structural highlights

1nh9 is a 1 chain structure with sequence from Methanocaldococcus jannaschii. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ALBA_METJA Binds double-stranded DNA tightly but without sequence specificity. It is distributed uniformly and abundantly on the chromosome, suggesting a role in chromatin architecture. However, it does not significantly compact DNA. Binds rRNA and mRNA in vivo. May play a role in maintaining the structural and functional stability of RNA, and, perhaps, ribosomes (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The Sac10b family consists of a group of highly conserved DNA binding proteins from both the euryarchaeotal and the crenarchaeotal branches of Archaea. The proteins have been suggested to play an architectural role in the chromosomal organization in these organisms. Previous studies have mainly focused on the Sac10b proteins from the crenarchaeota. Here, we report the 2.0 A resolution crystal structure of Mja10b from the euryarchaeon Methanococcus jannaschii. The model of Mja10b has been refined to an R-factor of 20.9%. The crystal structure of an Mja10b monomer reveals an alpha/beta structure of four beta-strands and two alpha-helices, and Mja10b assembles into a dimer via an extensive hydrophobic interface. Mja10b has a similar topology to that of its crenarchaeota counterpart Sso10b (also known as Alba). Structural comparison between the two proteins suggests that structural features such as hydrophobic inner core, acetylation sites, dimer interface, and DNA binding surface are conserved among Sac10b proteins. Structural differences between the two proteins were found in the loops. To understand the structural basis for the thermostability of Mja10b, the Mja10b structure was compared to other proteins with similar topology. Our data suggest that extensive ion-pair networks, optimized accessible surface area and the dimerization via hydrophobic interactions may contribute to the enhanced thermostability of Mja10b.

Crystal structure of a DNA binding protein from the hyperthermophilic euryarchaeon Methanococcus jannaschii.,Wang G, Guo R, Bartlam M, Yang H, Xue H, Liu Y, Huang L, Rao Z Protein Sci. 2003 Dec;12(12):2815-22. PMID:14627741^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Wang G, Guo R, Bartlam M, Yang H, Xue H, Liu Y, Huang L, Rao Z. Crystal structure of a DNA binding protein from the hyperthermophilic euryarchaeon Methanococcus jannaschii. Protein Sci. 2003 Dec;12(12):2815-22. PMID:14627741

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1nh9"

@@ Line 3: / Line 3: @@
 <StructureSection load='1nh9' size='340' side='right'caption='[[1nh9]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1nh9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_43067 Atcc 43067]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NH9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NH9 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1nh9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NH9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NH9 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nh9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nh9 OCA], [http://pdbe.org/1nh9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1nh9 RCSB], [http://www.ebi.ac.uk/pdbsum/1nh9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1nh9 ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nh9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nh9 OCA], [https://pdbe.org/1nh9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nh9 RCSB], [https://www.ebi.ac.uk/pdbsum/1nh9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nh9 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/ALBA_METJA ALBA_METJA]] Binds double-stranded DNA tightly but without sequence specificity. It is distributed uniformly and abundantly on the chromosome, suggesting a role in chromatin architecture. However, it does not significantly compact DNA. Binds rRNA and mRNA in vivo. May play a role in maintaining the structural and functional stability of RNA, and, perhaps, ribosomes (By similarity).
+[https://www.uniprot.org/uniprot/ALBA_METJA ALBA_METJA] Binds double-stranded DNA tightly but without sequence specificity. It is distributed uniformly and abundantly on the chromosome, suggesting a role in chromatin architecture. However, it does not significantly compact DNA. Binds rRNA and mRNA in vivo. May play a role in maintaining the structural and functional stability of RNA, and, perhaps, ribosomes (By similarity).
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 31: / Line 32: @@
 __TOC__
 </StructureSection>
-[[Category: Atcc 43067]]
 [[Category: Large Structures]]
-[[Category: Bartlam, M]]
+[[Category: Methanocaldococcus jannaschii]]
-[[Category: Guo, R]]
+[[Category: Bartlam M]]
-[[Category: Huang, L]]
+[[Category: Guo R]]
-[[Category: Liu, Y]]
+[[Category: Huang L]]
-[[Category: Rao, Z]]
+[[Category: Liu Y]]
-[[Category: Wang, G]]
+[[Category: Rao Z]]
-[[Category: Xue, H]]
+[[Category: Wang G]]
-[[Category: Yang, H]]
+[[Category: Xue H]]
-[[Category: Dna binding protein]]
+[[Category: Yang H]]
-[[Category: Mja10b]]

1nh9

From Proteopedia

Current revision

Crystal Structure of a DNA Binding Protein Mja10b from the hyperthermophile Methanococcus jannaschii

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox