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| | <StructureSection load='3e5v' size='340' side='right'caption='[[3e5v]], [[Resolution|resolution]] 2.10Å' scene=''> | | <StructureSection load='3e5v' size='340' side='right'caption='[[3e5v]], [[Resolution|resolution]] 2.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3e5v]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bubble-tip_anemone Bubble-tip anemone]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3E5V FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3e5v]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Entacmaea_quadricolor Entacmaea quadricolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3E5V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3E5V FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NRQ:{(4Z)-4-(4-HYDROXYBENZYLIDENE)-2-[3-(METHYLTHIO)PROPANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRQ</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3e5t|3e5t]], [[3e5w|3e5w]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NRQ:{(4Z)-4-(4-HYDROXYBENZYLIDENE)-2-[3-(METHYLTHIO)PROPANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRQ</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3e5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e5v OCA], [http://pdbe.org/3e5v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3e5v RCSB], [http://www.ebi.ac.uk/pdbsum/3e5v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3e5v ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3e5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3e5v OCA], [https://pdbe.org/3e5v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3e5v RCSB], [https://www.ebi.ac.uk/pdbsum/3e5v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3e5v ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/RFP_ENTQU RFP_ENTQU]] Pigment protein.<ref>PMID:12185250</ref> | + | [https://www.uniprot.org/uniprot/RFP_ENTQU RFP_ENTQU] Pigment protein.<ref>PMID:12185250</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bubble-tip anemone]] | + | [[Category: Entacmaea quadricolor]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Nar, H]] | + | [[Category: Nar H]] |
| - | [[Category: Nienhaus, K]] | + | [[Category: Nienhaus K]] |
| - | [[Category: Nienhaus, U]] | + | [[Category: Nienhaus U]] |
| - | [[Category: Wiedenmann, J]] | + | [[Category: Wiedenmann J]] |
| - | [[Category: Chromophore]]
| + | |
| - | [[Category: Cis-trans isomer]]
| + | |
| - | [[Category: D2rfp630]]
| + | |
| - | [[Category: Fluorescent protein]]
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| - | [[Category: Luminescence]]
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| - | [[Category: Photoprotein]]
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| - | [[Category: Red fluorescent protein]]
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| Structural highlights
Function
RFP_ENTQU Pigment protein.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
An important class of red fluorescent proteins (RFPs) feature a 2-iminomethyl-5-(4-hydroxybenzylidene)imidazolinone chromophore. Among these proteins, eqFP611 has the chromophore in a coplanar trans orientation, whereas the cis isomer is preferred by other RFPs such as DsRed and its variants. In the photoactivatable protein asFP595, the chromophore can even be switched from the nonfluorescent trans to the fluorescent cis state by light. By using X-ray crystallography, we have determined the structure of dimeric eqFP611 at high resolution (up to 1.1 A). In the far-red emitting eqFP611 variant d2RFP630, which carries an additional Asn143Ser mutation, the chromophore resides predominantly (approximately 80%) in the cis isomeric state, and in RFP639, which has Asn143Ser and Ser158Cys mutations, the chromophore is found completely in the cis form. The pronounced red shift of excitation and emission maxima of RFP639 can thus unambiguously be assigned to trans-cis isomerization of the chromophore. Among RFPs, eqFP611 is thus unique because its chromophore is highly fluorescent in both the cis and trans isomeric forms.
Trans-cis isomerization is responsible for the red-shifted fluorescence in variants of the red fluorescent protein eqFP611.,Nienhaus K, Nar H, Heilker R, Wiedenmann J, Nienhaus GU J Am Chem Soc. 2008 Sep 24;130(38):12578-9. Epub 2008 Aug 30. PMID:18761441[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Wiedenmann J, Schenk A, Rocker C, Girod A, Spindler KD, Nienhaus GU. A far-red fluorescent protein with fast maturation and reduced oligomerization tendency from Entacmaea quadricolor (Anthozoa, Actinaria). Proc Natl Acad Sci U S A. 2002 Sep 3;99(18):11646-51. Epub 2002 Aug 15. PMID:12185250 doi:http://dx.doi.org/10.1073/pnas.182157199
- ↑ Nienhaus K, Nar H, Heilker R, Wiedenmann J, Nienhaus GU. Trans-cis isomerization is responsible for the red-shifted fluorescence in variants of the red fluorescent protein eqFP611. J Am Chem Soc. 2008 Sep 24;130(38):12578-9. Epub 2008 Aug 30. PMID:18761441 doi:10.1021/ja8046443
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