3brk

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[[Image:3brk.jpg|left|200px]]
 
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==Crystal Structure of ADP-Glucose Pyrophosphorylase from Agrobacterium tumefaciens==
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The line below this paragraph, containing "STRUCTURE_3brk", creates the "Structure Box" on the page.
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<StructureSection load='3brk' size='340' side='right'caption='[[3brk]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3brk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Agrobacterium_tumefaciens Agrobacterium tumefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BRK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BRK FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_3brk| PDB=3brk | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3brk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3brk OCA], [https://pdbe.org/3brk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3brk RCSB], [https://www.ebi.ac.uk/pdbsum/3brk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3brk ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GLGC_RHIRD GLGC_RHIRD] Catalyzes the synthesis of ADP-glucose, a sugar donor used in elongation reactions on alpha-glucans.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/br/3brk_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3brk ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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ADP-glucose pyrophosphorylase (ADPGlc PPase) catalyzes the conversion of glucose 1-phosphate and ATP to ADP-glucose and pyrophosphate. As a key step in glucan synthesis, the ADPGlc PPases are highly regulated by allosteric activators and inhibitors in accord with the carbon metabolism pathways of the organism. Crystals of Agrobacterium tumefaciens ADPGlc PPase were obtained using lithium sulfate as a precipitant. A complete anomalous selenomethionyl derivative X-ray diffraction data set was collected with unit cell dimensions a = 85.38 A, b = 93.79 A, and c = 140.29 A (alpha = beta = gamma = 90 degrees ) and space group I 222. The A. tumefaciens ADPGlc PPase model was refined to 2.1 A with an R factor = 22% and R free = 26.6%. The model consists of two domains: an N-terminal alphabetaalpha sandwich and a C-terminal parallel beta-helix. ATP and glucose 1-phosphate were successfully modeled in the proposed active site, and site-directed mutagenesis of conserved glycines in this region (G20, G21, and G23) resulted in substantial loss of activity. The interface between the N- and the C-terminal domains harbors a strong sulfate-binding site, and kinetic studies revealed that sulfate is a competitive inhibitor for the allosteric activator fructose 6-phosphate. These results suggest that the interface between the N- and C-terminal domains binds the allosteric regulator, and fructose 6-phosphate was modeled into this region. The A. tumefaciens ADPGlc PPase/fructose 6-phosphate structural model along with sequence alignment analysis was used to design mutagenesis experiments to expand the activator specificity to include fructose 1,6-bisphosphate. The H379R and H379K enzymes were found to be activated by fructose 1,6-bisphosphate.
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'''Crystal Structure of ADP-Glucose Pyrophosphorylase from Agrobacterium tumefaciens'''
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Structural analysis of ADP-glucose pyrophosphorylase from the bacterium Agrobacterium tumefaciens.,Cupp-Vickery JR, Igarashi RY, Perez M, Poland M, Meyer CR Biochemistry. 2008 Apr 15;47(15):4439-51. Epub 2008 Mar 21. PMID:18355040<ref>PMID:18355040</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==About this Structure==
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</div>
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3BRK is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Agrobacterium_tumefaciens Agrobacterium tumefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BRK OCA].
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<div class="pdbe-citations 3brk" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Agrobacterium tumefaciens]]
[[Category: Agrobacterium tumefaciens]]
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[[Category: Glucose-1-phosphate adenylyltransferase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Cupp-Vickery J]]
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[[Category: Cupp-Vickery, J.]]
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[[Category: Igarashi R]]
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[[Category: Igarashi, R.]]
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[[Category: Meyer C]]
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[[Category: Meyer, C.]]
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[[Category: Adp-glucose pyrophosphorylase]]
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[[Category: Agrobacterium tumefacien]]
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[[Category: Allostery]]
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[[Category: Glycogen biosynthesis]]
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[[Category: Kinetic]]
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[[Category: Nucleotidyltransferase]]
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[[Category: Site-directed mutagenesis]]
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[[Category: Structure-function relationship]]
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[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr 24 09:49:01 2008''
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Current revision

Crystal Structure of ADP-Glucose Pyrophosphorylase from Agrobacterium tumefaciens

PDB ID 3brk

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