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6po6

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MicroED Structure of a Natural Product VFAThiaGlu

Structural highlights

6po6 is a 1 chain structure with sequence from Pseudomonas syringae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron crystallography, Resolution 1Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Genome sequencing of environmental bacteria allows identification of biosynthetic gene clusters encoding unusual combinations of enzymes that produce unknown natural products. We identified a pathway in which a ribosomally synthesized small peptide serves as a scaffold for nonribosomal peptide extension and chemical modification. Amino acids are transferred to the carboxyl terminus of the peptide through adenosine triphosphate and amino acyl-tRNA-dependent chemistry that is independent of the ribosome. Oxidative rearrangement, carboxymethylation, and proteolysis of a terminal cysteine yields an amino acid-derived small molecule. Microcrystal electron diffraction demonstrates that the resulting product is isosteric to glutamate. We show that a similar peptide extension is used during the biosynthesis of the ammosamides, which are cytotoxic pyrroloquinoline alkaloids. These results suggest an alternative paradigm for biosynthesis of amino acid-derived natural products.

Use of a scaffold peptide in the biosynthesis of amino acid-derived natural products.,Ting CP, Funk MA, Halaby SL, Zhang Z, Gonen T, van der Donk WA Science. 2019 Jul 19;365(6450):280-284. doi: 10.1126/science.aau6232. PMID:31320540^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ting CP, Funk MA, Halaby SL, Zhang Z, Gonen T, van der Donk WA. Use of a scaffold peptide in the biosynthesis of amino acid-derived natural products. Science. 2019 Jul 19;365(6450):280-284. doi: 10.1126/science.aau6232. PMID:31320540 doi:http://dx.doi.org/10.1126/science.aau6232

1 Structural highlights
2 Publication Abstract from PubMed
3 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6po6"

@@ Line 3: / Line 3: @@
 <StructureSection load='6po6' size='340' side='right'caption='[[6po6]], [[Resolution|resolution]] 1.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6po6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_syringae Pseudomonas syringae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PO6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6PO6 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6po6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_syringae Pseudomonas syringae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PO6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6PO6 FirstGlance]. <br>
-</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OV7:'>OV7</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron crystallography, [[Resolution|Resolution]] 1&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6po6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6po6 OCA], [http://pdbe.org/6po6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6po6 RCSB], [http://www.ebi.ac.uk/pdbsum/6po6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6po6 ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=OV7:(2R)-amino[(carboxymethyl)sulfanyl]acetic+acid'>OV7</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6po6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6po6 OCA], [https://pdbe.org/6po6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6po6 RCSB], [https://www.ebi.ac.uk/pdbsum/6po6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6po6 ProSAT]</span></td></tr>
 </table>
 <div style="background-color:#fffaf0;">
@@ Line 22: / Line 23: @@
 [[Category: Large Structures]]
 [[Category: Pseudomonas syringae]]
-[[Category: Donk, W A.van der]]
+[[Category: Funk MA]]
-[[Category: Funk, M A]]
+[[Category: Gonen T]]
-[[Category: Gonen, T]]
+[[Category: Halaby S]]
-[[Category: Halaby, S]]
+[[Category: Ting CP]]
-[[Category: Ting, C P]]
+[[Category: Van der Donk WA]]
-[[Category: Microcrystal electron diffraction]]
-[[Category: Microed]]
-[[Category: Ribosomal synthesized small peptide]]
-[[Category: Unknown function]]

6po6

From Proteopedia

Current revision

MicroED Structure of a Natural Product VFAThiaGlu

Structural highlights

Publication Abstract from PubMed

References

Contents

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