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| | <StructureSection load='3gzj' size='340' side='right'caption='[[3gzj]], [[Resolution|resolution]] 2.19Å' scene=''> | | <StructureSection load='3gzj' size='340' side='right'caption='[[3gzj]], [[Resolution|resolution]] 2.19Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3gzj]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Ophioxylon_serpentinum Ophioxylon serpentinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GZJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3GZJ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3gzj]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Rauvolfia_serpentina Rauvolfia serpentina]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3GZJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3GZJ FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EVS:16-EPI-VELLOSIMINE'>EVS</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.19Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Polyneuridine-aldehyde_esterase Polyneuridine-aldehyde esterase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.78 3.1.1.78] </span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EVS:16-EPI-VELLOSIMINE'>EVS</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3gzj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gzj OCA], [http://pdbe.org/3gzj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3gzj RCSB], [http://www.ebi.ac.uk/pdbsum/3gzj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3gzj ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3gzj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3gzj OCA], [https://pdbe.org/3gzj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3gzj RCSB], [https://www.ebi.ac.uk/pdbsum/3gzj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3gzj ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PNAE_RAUSE PNAE_RAUSE]] Catalyzes the hydrolysis of polyneuridine aldehyde into epi-vellosimine, which is the immediate precursor for the synthesis of the ajmaline.<ref>PMID:10691977</ref> | + | [https://www.uniprot.org/uniprot/PNAE_RAUSE PNAE_RAUSE] Catalyzes the hydrolysis of polyneuridine aldehyde into epi-vellosimine, which is the immediate precursor for the synthesis of the ajmaline.<ref>PMID:10691977</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Ophioxylon serpentinum]] | + | [[Category: Rauvolfia serpentina]] |
| - | [[Category: Polyneuridine-aldehyde esterase]]
| + | [[Category: Hill M]] |
| - | [[Category: Hill, M]] | + | [[Category: Panjikar S]] |
| - | [[Category: Panjikar, S]] | + | [[Category: Stoeckigt J]] |
| - | [[Category: Stoeckigt, J]] | + | [[Category: Wang M]] |
| - | [[Category: Wang, M]] | + | [[Category: Yang L]] |
| - | [[Category: Yang, L]] | + | |
| - | [[Category: Alkaloid metabolism]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Hydrolase superfamily]]
| + | |
| - | [[Category: Serine esterase]]
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| Structural highlights
Function
PNAE_RAUSE Catalyzes the hydrolysis of polyneuridine aldehyde into epi-vellosimine, which is the immediate precursor for the synthesis of the ajmaline.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Cutting carbons: The three-dimensional structure of polyneuridine aldehyde esterase (PNAE) gives insight into the enzymatic mechanism of the biosynthesis of C(9)- from C(10)-monoterpenoid indole alkaloids (see scheme). PNAE is a very substrate-specific serine esterase. It harbors the catalytic triad S87-D216-H244, and is a new member of the alpha/beta-fold hydrolase superfamily. Its novel function leads to the diversification of alkaloid structures.
Structural basis and enzymatic mechanism of the biosynthesis of C9- from C10-monoterpenoid indole alkaloids.,Yang L, Hill M, Wang M, Panjikar S, Stockigt J Angew Chem Int Ed Engl. 2009;48(28):5211-3. PMID:19496101[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Dogru E, Warzecha H, Seibel F, Haebel S, Lottspeich F, Stockigt J. The gene encoding polyneuridine aldehyde esterase of monoterpenoid indole alkaloid biosynthesis in plants is an ortholog of the alpha/betahydrolase super family. Eur J Biochem. 2000 Mar;267(5):1397-406. PMID:10691977
- ↑ Yang L, Hill M, Wang M, Panjikar S, Stockigt J. Structural basis and enzymatic mechanism of the biosynthesis of C9- from C10-monoterpenoid indole alkaloids. Angew Chem Int Ed Engl. 2009;48(28):5211-3. PMID:19496101 doi:10.1002/anie.200900150
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