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3vu4

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<StructureSection load='3vu4' size='340' side='right'caption='[[3vu4]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
<StructureSection load='3vu4' size='340' side='right'caption='[[3vu4]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3vu4]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cbs_712 Cbs 712]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VU4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VU4 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3vu4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Kluyveromyces_marxianus Kluyveromyces marxianus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VU4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3VU4 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vu4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vu4 OCA], [http://pdbe.org/3vu4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3vu4 RCSB], [http://www.ebi.ac.uk/pdbsum/3vu4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3vu4 ProSAT]</span></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3vu4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vu4 OCA], [https://pdbe.org/3vu4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3vu4 RCSB], [https://www.ebi.ac.uk/pdbsum/3vu4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3vu4 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/J3QW34_KLUMA J3QW34_KLUMA]
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Autophagy is an intracellular degradation system by which cytoplasmic materials are enclosed by an autophagosome and delivered to a lysosome/vacuole. Atg18 plays a critical role in autophagosome formation as a complex with Atg2 and phosphatidylinositol 3-phosphate (PtdIns(3)P). However, little is known about the structure of Atg18 and its recognition mode of Atg2 or PtdIns(3)P. Here, we report the crystal structure of Kluyveromyces marxianus Hsv2, an Atg18 paralog, at 2.6 A resolution. The structure reveals a seven-bladed beta-propeller without circular permutation. Mutational analyses of Atg18 based on the K. marxianus Hsv2 structure suggested that Atg18 has two phosphoinositide-binding sites at blades 5 and 6, whereas the Atg2-binding region is located at blade 2. Point mutations in the loops of blade 2 specifically abrogated autophagy without affecting another Atg18 function, the regulation of vacuolar morphology at the vacuolar membrane. This architecture enables Atg18 to form a complex with Atg2 and PtdIns(3)P in parallel, thereby functioning in the formation of autophagosomes at autophagic membranes.
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Structure-based analyses reveal distinct binding sites for Atg2 and phosphoinositides in Atg18.,Watanabe Y, Kobayashi T, Yamamoto H, Hoshida H, Akada R, Inagaki F, Ohsumi Y, Noda NN J Biol Chem. 2012 Sep 14;287(38):31681-90. doi: 10.1074/jbc.M112.397570. Epub, 2012 Jul 31. PMID:22851171<ref>PMID:22851171</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3vu4" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Cbs 712]]
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[[Category: Kluyveromyces marxianus]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Noda, N N]]
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[[Category: Noda NN]]
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[[Category: Watanabe, Y]]
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[[Category: Watanabe Y]]
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[[Category: Beta-propeller fold]]
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[[Category: Protein transport]]
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Current revision

Crystal structure of Kluyvelomyces marxianus Hsv2

PDB ID 3vu4

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