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| | ==Solution structure of antifungal protein NFAP== | | ==Solution structure of antifungal protein NFAP== |
| - | <StructureSection load='5oqs' size='340' side='right'caption='[[5oqs]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='5oqs' size='340' side='right'caption='[[5oqs]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5oqs]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_fischeri_nrrl_181 Aspergillus fischeri nrrl 181]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OQS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OQS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5oqs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_fischeri_NRRL_181 Aspergillus fischeri NRRL 181]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OQS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OQS FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NFIA_112130 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=331117 Aspergillus fischeri NRRL 181])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5oqs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oqs OCA], [http://pdbe.org/5oqs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5oqs RCSB], [http://www.ebi.ac.uk/pdbsum/5oqs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5oqs ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5oqs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oqs OCA], [https://pdbe.org/5oqs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5oqs RCSB], [https://www.ebi.ac.uk/pdbsum/5oqs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5oqs ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A1D8H8_NEOFI A1D8H8_NEOFI] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Aspergillus fischeri nrrl 181]] | + | [[Category: Aspergillus fischeri NRRL 181]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Batta, G]] | + | [[Category: Batta G]] |
| - | [[Category: Czajlik, A]] | + | [[Category: Czajlik A]] |
| - | [[Category: Galgoczy, L]] | + | [[Category: Galgoczy L]] |
| - | [[Category: Hajdu, D]] | + | [[Category: Hajdu D]] |
| - | [[Category: Marx, F]] | + | [[Category: Marx F]] |
| - | [[Category: Antifungal protein]]
| + | |
| - | [[Category: Nfap]]
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| - | [[Category: Solution structure]]
| + | |
| - | [[Category: Structure from cyana 2 1]]
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| Structural highlights
Function
A1D8H8_NEOFI
Publication Abstract from PubMed
Small, cysteine-rich and cationic antifungal proteins from natural sources are promising candidates for the development of novel treatment strategies to prevent and combat infections caused by drug-resistant fungi. However, limited information about their structure and antifungal mechanism hampers their future applications. In the present study, we determined the solution structure, dynamics and associated solvent areas of the Neosartorya (Aspergillus) fischeri antifungal protein NFAP. Genome mining within the genus revealed the presence of orthologous genes in N. fischeri and Neosartorya spathulata, and genes encoding closely related proteins can be found in Penicillium brasiliensis and Penicillium oxalicum. We show that the tertiary structure of these putative proteins can be resolved using the structure of NFAP as reliable template for in silico prediction. Localization studies with fluorescence-labelled protein pointed at an energy-dependent uptake mechanism of NFAP in the sensitive model fungus Neurospora crassa and subsequent cytoplasmic localization coincided with cell-death induction. The presented results contribute to a better understanding of the structure/function relationship of NFAP and related proteins and pave the way towards future antifungal drug development.
Solution structure and novel insights into phylogeny and mode of action of the Neosartorya (Aspergillus) fischeri antifungal protein (NFAP).,Hajdu D, Huber A, Czajlik A, Toth L, Kele Z, Kocsube S, Fizil A, Marx F, Galgoczy L, Batta G Int J Biol Macromol. 2019 May 15;129:511-522. doi:, 10.1016/j.ijbiomac.2019.02.016. Epub 2019 Feb 7. PMID:30738898[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Hajdu D, Huber A, Czajlik A, Toth L, Kele Z, Kocsube S, Fizil A, Marx F, Galgoczy L, Batta G. Solution structure and novel insights into phylogeny and mode of action of the Neosartorya (Aspergillus) fischeri antifungal protein (NFAP). Int J Biol Macromol. 2019 May 15;129:511-522. doi:, 10.1016/j.ijbiomac.2019.02.016. Epub 2019 Feb 7. PMID:30738898 doi:http://dx.doi.org/10.1016/j.ijbiomac.2019.02.016
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