3cra

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[[Image:3cra.jpg|left|200px]]
 
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==Crystal Structure of Escherichia coli MazG, the Regulator of Nutritional Stress Response==
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The line below this paragraph, containing "STRUCTURE_3cra", creates the "Structure Box" on the page.
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<StructureSection load='3cra' size='340' side='right'caption='[[3cra]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3cra]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CRA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CRA FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cra FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cra OCA], [https://pdbe.org/3cra PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cra RCSB], [https://www.ebi.ac.uk/pdbsum/3cra PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cra ProSAT]</span></td></tr>
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{{STRUCTURE_3cra| PDB=3cra | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/MAZG_ECOLI MAZG_ECOLI]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cr/3cra_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cra ConSurf].
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<div style="clear:both"></div>
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'''Crystal Structure of Escherichia coli MazG, the Regulator of Nutritional Stress Response'''
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==See Also==
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*[[Response regulator 3D structure|Response regulator 3D structure]]
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__TOC__
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==Overview==
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</StructureSection>
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MazG is a nucleoside triphosphate pyrophosphohydrolase that hydrolyzes all canonical nucleoside triphosphates. mazG gene located downstream from mazEF - chromosomal "addiction module", mediated programmed cell death in E. coli. MazG activity is inhibited by MazEF complex both in vivo and in vitro. Enzymatic activity of MazG in vivo affects the cellular level of guanosine 3',5'-bispyrophosphate (ppGpp), synthesized by RelA under amino-acid starvation. The reduction of ppGpp, caused by MazG, may extend the period of cell survival under nutritional stress. Here we describe the first crystal structure of active MazG from E. coli, which is composed of two similarly folded globular domains in tandem. Among two putative catalytic domains, only the Cterminal domain has well-ordered active sites and exhibits an NTPase activity, which are derived from the tightly formed 'additional region'. The MazG-ATP complex structure and subsequent mutagenesis studies explain the peculiar active site environment accommodating all eight canonical NTPs as substrates. In vivo nutrient starvation experiments show that the C-terminus NTPase activity is responsible for the regulation of bacterial cell survival under nutritional stress.
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[[Category: Escherichia coli K-12]]
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[[Category: Large Structures]]
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==About this Structure==
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[[Category: Kang BS]]
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3CRA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CRA OCA].
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[[Category: Kim JS]]
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[[Category: Kim KJ]]
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==Reference==
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[[Category: Kim MH]]
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Crystal structure of Escherichia coli MazG, the regulator of nutritional stress response., Lee S, Kim MH, Kang BS, Kim JS, Kim GH, Kim YG, Kim KJ, J Biol Chem. 2008 Mar 18;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18353782 18353782]
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[[Category: Kim YG]]
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[[Category: Escherichia coli]]
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[[Category: Lee S]]
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[[Category: Single protein]]
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[[Category: Kang, B S.]]
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[[Category: Kim, J S.]]
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[[Category: Kim, K J.]]
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[[Category: Kim, M H.]]
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[[Category: Kim, Y G.]]
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[[Category: Lee, S.]]
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[[Category: Hydrolase]]
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[[Category: Tandem-repeat domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr 24 09:55:07 2008''
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Current revision

Crystal Structure of Escherichia coli MazG, the Regulator of Nutritional Stress Response

PDB ID 3cra

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