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6nfu

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Structure of the KcsA-G77A mutant or the 2,4-ion bound configuration of a K+ channel selectivity filter.

Structural highlights

6nfu is a 3 chain structure with sequence from Mus musculus and Streptomyces lividans. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.09Å
Ligands:	, ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

KCSA_STRLI Acts as a pH-gated potassium ion channel; changing the cytosolic pH from 7 to 4 opens the channel, although it is not clear if this is the physiological stimulus for channel opening. Monovalent cation preference is K(+) > Rb(+) > NH4(+) >> Na(+) > Li(+).^[1]

Publication Abstract from PubMed

Here, we present the atomic resolution crystallographic structure, the function, and the ion-binding properties of the KcsA mutants, G77A and G77C, that stabilize the 2,4-ion-bound configuration (i.e., water, K(+), water, K(+)-ion-bound configuration) of the K(+) channel's selectivity filter. A full functional and thermodynamic characterization of the G77A mutant revealed wild-type-like ion selectivity and apparent K(+)-binding affinity, in addition to showing a lack of C-type inactivation gating and a marked reduction in its single-channel conductance. These structures validate, from a structural point of view, the notion that 2 isoenergetic ion-bound configurations coexist within a K(+) channel's selectivity filter, which fully agrees with the water-K(+)-ion-coupled transport detected by streaming potential measurements.

Structure, function, and ion-binding properties of a K(+) channel stabilized in the 2,4-ion-bound configuration.,Tilegenova C, Cortes DM, Jahovic N, Hardy E, Hariharan P, Guan L, Cuello LG Proc Natl Acad Sci U S A. 2019 Aug 6. pii: 1901888116. doi:, 10.1073/pnas.1901888116. PMID:31387976^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Schrempf H, Schmidt O, Kummerlen R, Hinnah S, Muller D, Betzler M, Steinkamp T, Wagner R. A prokaryotic potassium ion channel with two predicted transmembrane segments from Streptomyces lividans. EMBO J. 1995 Nov 1;14(21):5170-8. PMID:7489706
↑ Tilegenova C, Cortes DM, Jahovic N, Hardy E, Hariharan P, Guan L, Cuello LG. Structure, function, and ion-binding properties of a K(+) channel stabilized in the 2,4-ion-bound configuration. Proc Natl Acad Sci U S A. 2019 Aug 6. pii: 1901888116. doi:, 10.1073/pnas.1901888116. PMID:31387976 doi:http://dx.doi.org/10.1073/pnas.1901888116

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6nfu"

@@ Line 3: / Line 3: @@
 <StructureSection load='6nfu' size='340' side='right'caption='[[6nfu]], [[Resolution|resolution]] 2.09&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6nfu]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_lividans"_krasil'nikov_et_al._1965 "actinomyces lividans" krasil'nikov et al. 1965] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NFU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NFU FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6nfu]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Streptomyces_lividans Streptomyces lividans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NFU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NFU FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1EM:(1S)-2-HYDROXY-1-[(NONANOYLOXY)METHYL]ETHYL+MYRISTATE'>1EM</scene>, <scene name='pdbligand=F09:NONAN-1-OL'>F09</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.09&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">kcsA, skc1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1916 "Actinomyces lividans" Krasil'nikov et al. 1965])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1EM:(1S)-2-HYDROXY-1-[(NONANOYLOXY)METHYL]ETHYL+MYRISTATE'>1EM</scene>, <scene name='pdbligand=F09:NONAN-1-OL'>F09</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6nfu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nfu OCA], [http://pdbe.org/6nfu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6nfu RCSB], [http://www.ebi.ac.uk/pdbsum/6nfu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6nfu ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6nfu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nfu OCA], [https://pdbe.org/6nfu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6nfu RCSB], [https://www.ebi.ac.uk/pdbsum/6nfu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6nfu ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/KCSA_STRLI KCSA_STRLI]] Acts as a pH-gated potassium ion channel; changing the cytosolic pH from 7 to 4 opens the channel, although it is not clear if this is the physiological stimulus for channel opening. Monovalent cation preference is K(+) > Rb(+) > NH4(+) >> Na(+) > Li(+).<ref>PMID:7489706</ref>
+[https://www.uniprot.org/uniprot/KCSA_STRLI KCSA_STRLI] Acts as a pH-gated potassium ion channel; changing the cytosolic pH from 7 to 4 opens the channel, although it is not clear if this is the physiological stimulus for channel opening. Monovalent cation preference is K(+) > Rb(+) > NH4(+) >> Na(+) > Li(+).<ref>PMID:7489706</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 19: / Line 19: @@
 </div>
 <div class="pdbe-citations 6nfu" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Antibody 3D structures|Antibody 3D structures]]
+*[[Potassium channel 3D structures|Potassium channel 3D structures]]
+*[[3D structures of non-human antibody|3D structures of non-human antibody]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Actinomyces lividans krasil'nikov et al. 1965]]
 [[Category: Large Structures]]
-[[Category: Lk3 transgenic mice]]
+[[Category: Mus musculus]]
-[[Category: Cortes, D M]]
+[[Category: Streptomyces lividans]]
-[[Category: Cuello, L G]]
+[[Category: Cortes DM]]
-[[Category: Guan, L]]
+[[Category: Cuello LG]]
-[[Category: Hardy, E]]
+[[Category: Guan L]]
-[[Category: Jahovic, N]]
+[[Category: Hardy E]]
-[[Category: Parameswaran, H]]
+[[Category: Jahovic N]]
-[[Category: Tilegenova, C]]
+[[Category: Parameswaran H]]
-[[Category: Ion channel]]
+[[Category: Tilegenova C]]
-[[Category: Membrane protein]]
-[[Category: Membrane transport]]
-[[Category: Metal transport]]
-[[Category: Potassium channel]]

6nfu

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Current revision

Structure of the KcsA-G77A mutant or the 2,4-ion bound configuration of a K+ channel selectivity filter.

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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