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| | ==N6-Methyladenosine RNA== | | ==N6-Methyladenosine RNA== |
| - | <StructureSection load='2mvs' size='340' side='right'caption='[[2mvs]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''> | + | <StructureSection load='2mvs' size='340' side='right'caption='[[2mvs]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2mvs]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MVS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MVS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2mvs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MVS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MVS FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=6MZ:N6-METHYLADENOSINE-5-MONOPHOSPHATE'>6MZ</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4rlp|4rlp]], [[2mvy|2mvy]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6MZ:N6-METHYLADENOSINE-5-MONOPHOSPHATE'>6MZ</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mvs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mvs OCA], [http://pdbe.org/2mvs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mvs RCSB], [http://www.ebi.ac.uk/pdbsum/2mvs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2mvs ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mvs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mvs OCA], [https://pdbe.org/2mvs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mvs RCSB], [https://www.ebi.ac.uk/pdbsum/2mvs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mvs ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Kool, E]] | + | [[Category: Synthetic construct]] |
| - | [[Category: Lynch, S R]] | + | [[Category: Kool E]] |
| - | [[Category: N6-methyl adenosine]] | + | [[Category: Lynch SR]] |
| - | [[Category: Rna]]
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| Structural highlights
Publication Abstract from PubMed
N(6)-Methyladenosine (m(6)A) modification is hypothesized to control processes such as RNA degradation, localization, and splicing. However, the molecular mechanisms by which this occurs are unclear. Here, we measured structures of an RNA duplex containing m(6)A in the GGACU consensus, along with an unmodified RNA control, by 2D NMR. The data show that m(6)A-U pairing in the double-stranded context is accompanied by the methylamino group rotating from its energetically preferred syn geometry on the Watson-Crick face to the higher-energy anti conformation, positioning the methyl group in the major groove. Thermodynamic measurements of m(6)A in duplexes reveal that it is destabilizing by 0.5-1.7 kcal/mol. In contrast, we show that m(6)A in unpaired positions base stacks considerably more strongly than the unmodified base, adding substantial stabilization in single-stranded locations. Transcriptome-wide nuclease mapping of methylated RNA secondary structure from human cells reveals a structural transition at methylated adenosines, with a tendency to single-stranded structure adjacent to the modified base.
Structure and thermodynamics of N6-methyladenosine in RNA: a spring-loaded base modification.,Roost C, Lynch SR, Batista PJ, Qu K, Chang HY, Kool ET J Am Chem Soc. 2015 Feb 11;137(5):2107-15. doi: 10.1021/ja513080v. Epub 2015 Feb , 2. PMID:25611135[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Roost C, Lynch SR, Batista PJ, Qu K, Chang HY, Kool ET. Structure and thermodynamics of N6-methyladenosine in RNA: a spring-loaded base modification. J Am Chem Soc. 2015 Feb 11;137(5):2107-15. doi: 10.1021/ja513080v. Epub 2015 Feb , 2. PMID:25611135 doi:http://dx.doi.org/10.1021/ja513080v
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