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| | ==NMR STRUCTURES OF WHEAT NONSPECIFIC LIPID TRANSFER PROTEIN== | | ==NMR STRUCTURES OF WHEAT NONSPECIFIC LIPID TRANSFER PROTEIN== |
| - | <StructureSection load='1gh1' size='340' side='right'caption='[[1gh1]], [[NMR_Ensembles_of_Models | 15 NMR models]]' scene=''> | + | <StructureSection load='1gh1' size='340' side='right'caption='[[1gh1]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1gh1]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1lpt 1lpt]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GH1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GH1 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1gh1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Triticum_aestivum Triticum aestivum]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1lpt 1lpt]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GH1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GH1 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1cz2|1cz2]], [[1bwo|1bwo]], [[1azh|1azh]], [[1mzl|1mzl]], [[1afh|1afh]], [[1mzm|1mzm]], [[1lip|1lip]], [[1jtb|1jtb]], [[1be2|1be2]], [[1bv2|1bv2]], [[1rzl|1rzl]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1gh1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gh1 OCA], [http://pdbe.org/1gh1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1gh1 RCSB], [http://www.ebi.ac.uk/pdbsum/1gh1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1gh1 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gh1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gh1 OCA], [https://pdbe.org/1gh1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gh1 RCSB], [https://www.ebi.ac.uk/pdbsum/1gh1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gh1 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/NLTP1_WHEAT NLTP1_WHEAT]] Plant non-specific lipid-transfer proteins transfer phospholipids as well as galactolipids across membranes. May play a role in wax or cutin deposition in the cell walls of expanding epidermal cells and certain secretory tissues. | + | [https://www.uniprot.org/uniprot/NLTP1_WHEAT NLTP1_WHEAT] Plant non-specific lipid-transfer proteins transfer phospholipids as well as galactolipids across membranes. May play a role in wax or cutin deposition in the cell walls of expanding epidermal cells and certain secretory tissues. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| | [[Category: Triticum aestivum]] | | [[Category: Triticum aestivum]] |
| - | [[Category: Caille, A]] | + | [[Category: Caille A]] |
| - | [[Category: Gincel, E]] | + | [[Category: Gincel E]] |
| - | [[Category: Marion, D]] | + | [[Category: Marion D]] |
| - | [[Category: Ptak, M]] | + | [[Category: Ptak M]] |
| - | [[Category: Simorre, J P]] | + | [[Category: Simorre JP]] |
| - | [[Category: Vovelle, F]] | + | [[Category: Vovelle F]] |
| - | [[Category: 4-helix winding]]
| + | |
| - | [[Category: Lipid binding protein]]
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| Structural highlights
Function
NLTP1_WHEAT Plant non-specific lipid-transfer proteins transfer phospholipids as well as galactolipids across membranes. May play a role in wax or cutin deposition in the cell walls of expanding epidermal cells and certain secretory tissues.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Two-dimensional and three-dimensional 1H-NMR experimental data [Simorre, J. P., Caille, A., Marion, D., Marion, D. & Ptak, M. (1991) Biochemistry 30, 11600-11608] were used to build models of the three-dimensional structure of a non-specific wheat lipid-transfer protein (LTP) by using distance geometry, simulated annealing, energy minimization and molecular dynamics techniques. A first set of 881 distance constraints derived from NOE cross-peak intensities was used to generate 74 initial structures. One family of topological mirror images of the protein structure was eliminated by considering helical secondary-structure organization and steric requirements. Back calculations of NOE intensities led us to introduce 535 additional distance constraints. Finally, 21 structures were selected as representative of the structure of the protein. The polypeptide backbone folds into a simple and original right-handed winding. It is composed of a bundle of four helices linked by flexible loops, which is packed against a C-terminal fragment forming a non-standard saxophone-like shape. The folded protein is stabilized by hydrophobic interactions and the four disulfide bridges combined by pairs on each side of the protein. An hydrophobic cleft, formed by residues located in the second half of the protein could be a potential site for the binding of lipids.
Three-dimensional structure in solution of a wheat lipid-transfer protein from multidimensional 1H-NMR data. A new folding for lipid carriers.,Gincel E, Simorre JP, Caille A, Marion D, Ptak M, Vovelle F Eur J Biochem. 1994 Dec 1;226(2):413-22. PMID:8001559[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Gincel E, Simorre JP, Caille A, Marion D, Ptak M, Vovelle F. Three-dimensional structure in solution of a wheat lipid-transfer protein from multidimensional 1H-NMR data. A new folding for lipid carriers. Eur J Biochem. 1994 Dec 1;226(2):413-22. PMID:8001559
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