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| | <StructureSection load='3da0' size='340' side='right'caption='[[3da0]], [[Resolution|resolution]] 1.65Å' scene=''> | | <StructureSection load='3da0' size='340' side='right'caption='[[3da0]], [[Resolution|resolution]] 1.65Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3da0]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DA0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3DA0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3da0]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Lactococcus_phage_TP901-1 Lactococcus phage TP901-1] and [https://en.wikipedia.org/wiki/Lactococcus_virus_P2 Lactococcus virus P2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3DA0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3DA0 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1zru|1zru]], [[2bsd|2bsd]], [[2bse|2bse]], [[2f0c|2f0c]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3da0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3da0 OCA], [http://pdbe.org/3da0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3da0 RCSB], [http://www.ebi.ac.uk/pdbsum/3da0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3da0 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3da0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3da0 OCA], [https://pdbe.org/3da0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3da0 RCSB], [https://www.ebi.ac.uk/pdbsum/3da0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3da0 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q9G096_9CAUD Q9G096_9CAUD] [https://www.uniprot.org/uniprot/RBP_BPLP2 RBP_BPLP2] Binds to the host phosphopolysaccharides at the onset of infection. Upon activation by calcium, the receptor binding proteins change their conformation, presenting their binding sites to the host, and a channel opens at the bottom of the baseplate for DNA ejection.<ref>PMID:20351260</ref> <ref>PMID:24027307</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Lactococcus phage TP901-1]] |
| | + | [[Category: Lactococcus virus P2]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Blangy, S]] | + | [[Category: Blangy S]] |
| - | [[Category: Cambillau, C]] | + | [[Category: Cambillau C]] |
| - | [[Category: Campanacci, V]] | + | [[Category: Campanacci V]] |
| - | [[Category: Siponen, M I]] | + | [[Category: Siponen MI]] |
| - | [[Category: Spinelli, S]] | + | [[Category: Spinelli S]] |
| - | [[Category: Vera, L]] | + | [[Category: Vera L]] |
| - | [[Category: Lactococcal phage p2]]
| + | |
| - | [[Category: Lactococcal phage tp901-1 receptor binding protein]]
| + | |
| - | [[Category: Viral protein]]
| + | |
| Structural highlights
Function
Q9G096_9CAUD RBP_BPLP2 Binds to the host phosphopolysaccharides at the onset of infection. Upon activation by calcium, the receptor binding proteins change their conformation, presenting their binding sites to the host, and a channel opens at the bottom of the baseplate for DNA ejection.[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Lactococcus lactis, a gram-positive bacterium widely used by the dairy industry to manufacture cheeses, is subject to infection by a diverse population of virulent phages. We have previously determined the structures of three receptor binding proteins (RBPs) from lactococcal phages TP901-1, p2, and bIL170, each of them having a distinct host range. Virulent phages p2 and bIL170 are classified within the 936 group, while the temperate phage TP901-1 is a member of the genetically distinct P335 polythetic group. These RBPs comprise three domains: the N-terminal domain, binding to the virion particle; a beta-helical linker domain; and the C-terminal domain, bearing the receptor binding site used for host recognition. Here, we have designed, expressed, and determined the structure of an RBP chimera in which the N-terminal and linker RBP domains of phage TP901-1 (P335) are fused to the C-terminal RBP domain of phage p2 (936). This chimera exhibits a stable structure that closely resembles the parental structures, while a slight displacement of the linker made RBP domain adaptation efficient. The receptor binding site is structurally indistinguishable from that of native p2 RBP and binds glycerol with excellent affinity.
Crystal structure of a chimeric receptor binding protein constructed from two lactococcal phages.,Siponen M, Spinelli S, Blangy S, Moineau S, Cambillau C, Campanacci V J Bacteriol. 2009 May;191(10):3220-5. Epub 2009 Mar 13. PMID:19286807[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Sciara G, Bebeacua C, Bron P, Tremblay D, Ortiz-Lombardia M, Lichiere J, van Heel M, Campanacci V, Moineau S, Cambillau C. Structure of lactococcal phage p2 baseplate and its mechanism of activation. Proc Natl Acad Sci U S A. 2010 Mar 29. PMID:20351260
- ↑ Bebeacua C, Tremblay D, Farenc C, Chapot-Chartier MP, Sadovskaya I, van Heel M, Veesler D, Moineau S, Cambillau C. Structure, adsorption to host, and infection mechanism of virulent lactococcal phage p2. J Virol. 2013 Nov;87(22):12302-12. PMID:24027307 doi:10.1128/JVI.02033-13
- ↑ Siponen M, Spinelli S, Blangy S, Moineau S, Cambillau C, Campanacci V. Crystal structure of a chimeric receptor binding protein constructed from two lactococcal phages. J Bacteriol. 2009 May;191(10):3220-5. Epub 2009 Mar 13. PMID:19286807 doi:10.1128/JB.01637-08
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