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| | ==NMR STRUCTURE OF SPHERULIN 3A (S3A) FROM PHYSARUM POLYCEPHALUM, MINIMIZED AVERAGE STRUCTURE== | | ==NMR STRUCTURE OF SPHERULIN 3A (S3A) FROM PHYSARUM POLYCEPHALUM, MINIMIZED AVERAGE STRUCTURE== |
| - | <StructureSection load='1ag4' size='340' side='right'caption='[[1ag4]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''> | + | <StructureSection load='1ag4' size='340' side='right'caption='[[1ag4]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1ag4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Phypo Phypo]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AG4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1AG4 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1ag4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Physarum_polycephalum Physarum polycephalum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AG4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AG4 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ag4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ag4 OCA], [http://pdbe.org/1ag4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ag4 RCSB], [http://www.ebi.ac.uk/pdbsum/1ag4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ag4 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ag4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ag4 OCA], [https://pdbe.org/1ag4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ag4 RCSB], [https://www.ebi.ac.uk/pdbsum/1ag4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ag4 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/SR3A_PHYPO SR3A_PHYPO]] Structural protein. | + | [https://www.uniprot.org/uniprot/SR3A_PHYPO SR3A_PHYPO] Structural protein. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Phypo]] | + | [[Category: Physarum polycephalum]] |
| - | [[Category: Holak, T A]] | + | [[Category: Holak TA]] |
| - | [[Category: Jaenicke, R]] | + | [[Category: Jaenicke R]] |
| - | [[Category: Mayr, E M]] | + | [[Category: Mayr E-M]] |
| - | [[Category: Renner, C]] | + | [[Category: Renner C]] |
| - | [[Category: Rosinke, B]] | + | [[Category: Rosinke B]] |
| - | [[Category: Spherulation-specific protein]]
| + | |
| - | [[Category: Structural protein]]
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| Structural highlights
Function
SR3A_PHYPO Structural protein.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Spherulin 3a is the most abundantly expressed cytosolic protein in spherulating plasmodia of the slime mold Physarum polycephalum. High yields of unlabeled, uniformly 15N and uniformly 13C/15N-labeled recombinant spherulin 3a from Escherichia coli could be produced by a simple protocol described here. The three-dimensional solution structure of Ca2+-loaded spherulin 3a was determined by homo- and heteronuclear NMR spectroscopy. The structure of monomeric spherulin 3a consists of two pleated beta-sheets plus a short alpha-helix arranged into the gamma-crystallin fold. The beta-sheets comprise two intertwined Greek-key motifs. An additional N-terminal beta-strand is unique to spherulin 3a. Complexation of calcium ions greatly enhances overall conformational stability of the protein. The average atomic root-mean-square deviations (r.m.s.d.) for heavy atoms in beta-strands were 0.34(+/-0.16) A for the backbone atoms and 0.73(+/-0.40) A for all atoms. The corresponding r.m.s.d. values for heavy atoms in the whole protein were 0.62(+/-0.42) A for the backbone atoms and 0.99(+/-0.65) A for all atoms. We show the structural relationship between spherulin 3a, a myxomycete dormancy protein, and crystallins from the vertebrate eye lens. Since spherulin 3a has a structure corresponding to one domain of bovine gammaB(II)-crystallin, it represents a hypothetical ancestral gamma-crystallin precursor structure.
Ca2+-loaded spherulin 3a from Physarum polycephalum adopts the prototype gamma-crystallin fold in aqueous solution.,Rosinke B, Renner C, Mayr EM, Jaenicke R, Holak TA J Mol Biol. 1997 Aug 29;271(4):645-55. PMID:9281431[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Rosinke B, Renner C, Mayr EM, Jaenicke R, Holak TA. Ca2+-loaded spherulin 3a from Physarum polycephalum adopts the prototype gamma-crystallin fold in aqueous solution. J Mol Biol. 1997 Aug 29;271(4):645-55. PMID:9281431 doi:10.1006/jmbi.1997.1184
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