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2j7n

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Structure of the RNAi polymerase from Neurospora crassa

Structural highlights

2j7n is a 2 chain structure with sequence from Neurospora crassa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.3Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9Y7G6_NEUCS

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

RNA silencing refers to a group of RNA-induced gene-silencing mechanisms that developed early in the eukaryotic lineage, probably for defence against pathogens and regulation of gene expression. In plants, protozoa, fungi, and nematodes, but apparently not insects and vertebrates, it involves a cell-encoded RNA-dependent RNA polymerase (cRdRP) that produces double-stranded RNA triggers from aberrant single-stranded RNA. We report the 2.3-A resolution crystal structure of QDE-1, a cRdRP from Neurospora crassa, and find that it forms a relatively compact dimeric molecule, each subunit of which comprises several domains with, at its core, a catalytic apparatus and protein fold strikingly similar to the catalytic core of the DNA-dependent RNA polymerases responsible for transcription. This evolutionary link between the two enzyme types suggests that aspects of RNA silencing in some organisms may recapitulate transcription/replication pathways functioning in the ancient RNA-based world.

The structure of an RNAi polymerase links RNA silencing and transcription.,Salgado PS, Koivunen MR, Makeyev EV, Bamford DH, Stuart DI, Grimes JM PLoS Biol. 2006 Dec;4(12):e434. PMID:17147473^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Salgado PS, Koivunen MR, Makeyev EV, Bamford DH, Stuart DI, Grimes JM. The structure of an RNAi polymerase links RNA silencing and transcription. PLoS Biol. 2006 Dec;4(12):e434. PMID:17147473 doi:10.1371/journal.pbio.0040434

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2j7n"

@@ Line 3: / Line 3: @@
 <StructureSection load='2j7n' size='340' side='right'caption='[[2j7n]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2j7n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chrysonilia_crassa Chrysonilia crassa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J7N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2J7N FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2j7n]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Neurospora_crassa Neurospora crassa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J7N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J7N FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2j7o|2j7o]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j7n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j7n OCA], [http://pdbe.org/2j7n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2j7n RCSB], [http://www.ebi.ac.uk/pdbsum/2j7n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2j7n ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j7n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j7n OCA], [https://pdbe.org/2j7n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j7n RCSB], [https://www.ebi.ac.uk/pdbsum/2j7n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j7n ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q9Y7G6_NEUCS Q9Y7G6_NEUCS]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 29: / Line 31: @@
 ==See Also==
-*[[RNA polymerase|RNA polymerase]]
+*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Chrysonilia crassa]]
 [[Category: Large Structures]]
-[[Category: Bamford, D H]]
+[[Category: Neurospora crassa]]
-[[Category: Grimes, J M]]
+[[Category: Bamford DH]]
-[[Category: Koivunen, M R.L]]
+[[Category: Grimes JM]]
-[[Category: Makeyev, E V]]
+[[Category: Koivunen MRL]]
-[[Category: Salgado, P S]]
+[[Category: Makeyev EV]]
-[[Category: Stuart, D I]]
+[[Category: Salgado PS]]
-[[Category: Hydrolase]]
+[[Category: Stuart DI]]
-[[Category: Rna-directed rna polymerase]]
-[[Category: Rnai response]]

2j7n

From Proteopedia

Current revision

Structure of the RNAi polymerase from Neurospora crassa

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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