2r1j

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[[Image:2r1j.jpg|left|200px]]
 
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==Crystal Structure of the P22 c2 Repressor protein in complex with the synthetic operator 9T==
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The line below this paragraph, containing "STRUCTURE_2r1j", creates the "Structure Box" on the page.
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<StructureSection load='2r1j' size='340' side='right'caption='[[2r1j]], [[Resolution|resolution]] 1.53&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2r1j]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_virus_P22 Salmonella virus P22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R1J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2R1J FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.53&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2r1j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r1j OCA], [https://pdbe.org/2r1j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2r1j RCSB], [https://www.ebi.ac.uk/pdbsum/2r1j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2r1j ProSAT]</span></td></tr>
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{{STRUCTURE_2r1j| PDB=2r1j | SCENE= }}
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</table>
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== Function ==
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'''Crystal Structure of the P22 c2 Repressor protein in complex with the synthetic operator 9T'''
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[https://www.uniprot.org/uniprot/RPC2_BPP22 RPC2_BPP22] This protein allows the phage to reside inactively in the chromosome of its host bacterium. This lysogenic state is maintained by binding of regulatory protein C2 to the OR and OL operators, preventing transcription of proteins necessary for lytic development.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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The P22 c2 repressor protein (P22R) binds to DNA sequence-specifically and helps to direct the temperate lambdoid bacteriophage P22 to the lysogenic developmental pathway. We describe the 1.6 A X-ray structure of the N-terminal domain (NTD) of P22R in a complex with a DNA fragment containing the synthetic operator sequence [d(ATTTAAGATATCTTAAAT)]2. This operator has an A-T base pair at position 9L and a T-A base pair at position 9R and is termed DNA9T. Direct readout: nondirectional van der Waals interactions between protein and DNA appear to confer sequence-specificity. The structure of the P22R NTD-DNA9T complex suggests that sequence-specificity arises substantially from lock-and-key interaction of a valine with a complementary binding cleft on the major groove surface of DNA9T. The cleft is formed by four methyl groups on sequential base pairs of 5'-TTAA-3'. The valine cleft is intrinsic to the DNA sequence and does not arise from protein-induced DNA conformational changes. Protein-DNA hydrogen bonding plays a secondary role in specificity. Indirect readout: it is known that the noncontacted bases in the center of the complex are important determinants of affinity. The protein induces a transition of the noncontacted region from B-DNA to B'-DNA. The B' state is characterized by a narrow minor groove and a zigzag spine of hydration. The free energy of the transition from B- to B'-DNA is known to depend on the sequence. Thus, the observed DNA conformation and hydration allows for the formulation of a predictive model of the indirect readout phenomenon.
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r1/2r1j_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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2R1J is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p22 Enterobacteria phage p22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R1J OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2r1j ConSurf].
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P22 c2 repressor-operator complex: mechanisms of direct and indirect readout., Watkins D, Hsiao C, Woods KK, Koudelka GB, Williams LD, Biochemistry. 2008 Feb 26;47(8):2325-38. Epub 2008 Feb 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18237194 18237194]
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<div style="clear:both"></div>
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[[Category: Enterobacteria phage p22]]
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__TOC__
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[[Category: Single protein]]
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</StructureSection>
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[[Category: Hsiao, C.]]
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[[Category: Large Structures]]
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[[Category: Koudelka, G B.]]
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[[Category: Salmonella virus P22]]
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[[Category: Watkins, D.]]
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[[Category: Hsiao C]]
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[[Category: Williams, L D.]]
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[[Category: Koudelka GB]]
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[[Category: Woods, K.]]
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[[Category: Watkins D]]
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[[Category: Dna-binding]]
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[[Category: Williams LD]]
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[[Category: Helix-turn-helix]]
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[[Category: Woods K]]
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[[Category: Protein-dna complex]]
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[[Category: Repressor]]
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[[Category: Transcription]]
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[[Category: Transcription regulation]]
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[[Category: Transcription/dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 30 13:20:56 2008''
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Current revision

Crystal Structure of the P22 c2 Repressor protein in complex with the synthetic operator 9T

PDB ID 2r1j

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