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| | <StructureSection load='3rwt' size='340' side='right'caption='[[3rwt]], [[Resolution|resolution]] 3.00Å' scene=''> | | <StructureSection load='3rwt' size='340' side='right'caption='[[3rwt]], [[Resolution|resolution]] 3.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3rwt]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Bubble-tip_anemone Bubble-tip anemone]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RWT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RWT FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3rwt]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Entacmaea_quadricolor Entacmaea quadricolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RWT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RWT FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NRQ:{(4Z)-4-(4-HYDROXYBENZYLIDENE)-2-[3-(METHYLTHIO)PROPANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRQ</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NRQ:{(4Z)-4-(4-HYDROXYBENZYLIDENE)-2-[3-(METHYLTHIO)PROPANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRQ</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3rwa|3rwa]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rwt OCA], [https://pdbe.org/3rwt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rwt RCSB], [https://www.ebi.ac.uk/pdbsum/3rwt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rwt ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rwt OCA], [http://pdbe.org/3rwt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3rwt RCSB], [http://www.ebi.ac.uk/pdbsum/3rwt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3rwt ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/D0VX33_ENTQU D0VX33_ENTQU] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bubble-tip anemone]] | + | [[Category: Entacmaea quadricolor]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Sondermann, H]] | + | [[Category: Sondermann H]] |
| - | [[Category: Wang, Q]] | + | [[Category: Wang Q]] |
| - | [[Category: Circular permutated]]
| + | |
| - | [[Category: Fluorescent protein]]
| + | |
| - | [[Category: Gfp-like fluorescent protein]]
| + | |
| - | [[Category: Mkate]]
| + | |
| - | [[Category: Red fluorescentprotein]]
| + | |
| Structural highlights
Function
D0VX33_ENTQU
Publication Abstract from PubMed
Circular permutation of fluorescent proteins provides a substrate for the design of molecular sensors. Here we describe a systematic exploration of permutation sites for mCherry and mKate using a tandem fusion template approach. Circular permutants retaining more than 60% (mCherry) and 90% (mKate) brightness of the parent molecules are reported, as well as a quantitative evaluation of the fluorescence from neighboring mutations. Truncations of circular permutants indicated essential N- and C-terminal segments and substantial flexibility in the use of these molecules. Structural evaluation of two cp-mKate variants indicated no major conformational changes from the previously reported wild-type structure, and cis conformation of the chromophores. Four cp-mKates were identified with over 80% of native fluorescence, providing important new building blocks for sensor and complementation experiments.
Circular permutation of red fluorescent proteins.,Shui B, Wang Q, Lee F, Byrnes LJ, Chudakov DM, Lukyanov SA, Sondermann H, Kotlikoff MI PLoS One. 2011;6(5):e20505. Epub 2011 May 27. PMID:21647365[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Shui B, Wang Q, Lee F, Byrnes LJ, Chudakov DM, Lukyanov SA, Sondermann H, Kotlikoff MI. Circular permutation of red fluorescent proteins. PLoS One. 2011;6(5):e20505. Epub 2011 May 27. PMID:21647365 doi:10.1371/journal.pone.0020505
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