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| | <StructureSection load='3wcr' size='340' side='right'caption='[[3wcr]], [[Resolution|resolution]] 2.45Å' scene=''> | | <StructureSection load='3wcr' size='340' side='right'caption='[[3wcr]], [[Resolution|resolution]] 2.45Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3wcr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Phaseolus_vulgaris Phaseolus vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WCR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3WCR FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3wcr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Phaseolus_vulgaris Phaseolus vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3WCR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3WCR FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3wcs|3wcs]], [[3wog|3wog]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3wcr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wcr OCA], [http://pdbe.org/3wcr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3wcr RCSB], [http://www.ebi.ac.uk/pdbsum/3wcr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3wcr ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3wcr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3wcr OCA], [https://pdbe.org/3wcr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3wcr RCSB], [https://www.ebi.ac.uk/pdbsum/3wcr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3wcr ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/PHAE_PHAVU PHAE_PHAVU] This insecticidal carbohydrate-binding lectin is toxic for the cowpea weevil. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| | [[Category: Phaseolus vulgaris]] | | [[Category: Phaseolus vulgaris]] |
| - | [[Category: Nagae, M]] | + | [[Category: Nagae M]] |
| - | [[Category: Yamaguchi, Y]] | + | [[Category: Yamaguchi Y]] |
| - | [[Category: Carbohydrate binding]]
| + | |
| - | [[Category: Legume lectin fold]]
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| - | [[Category: N-glycan]]
| + | |
| - | [[Category: Sugar binding protein]]
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| Structural highlights
Function
PHAE_PHAVU This insecticidal carbohydrate-binding lectin is toxic for the cowpea weevil.
Publication Abstract from PubMed
Phytohemagglutinin from Phaseolus vulgaris (PHA-E), a legume lectin, has an unusual specificity toward biantennary galactosylated N-glycan with bisecting N-acetylglucosamine (GlcNAc). To investigate the interaction in detail, we have solved the crystal structures of PHA-E without ligand and in complex with biantennary N-glycan derivatives. PHA-E interacts with the trisaccharide unit (Galbeta1-4GlcNAcbeta1-2Man) in a manner completely different from that of mannose/glucose-specific legume lectins. The inner mannose residue binds to a novel site on the protein, and its rotation is opposite to that occurring in the monosaccharide-binding site of other lectins around the sugar O3 axis. Saturation-transfer difference NMR using biantennary di-galactosylated and bisected glycans reveals that PHA-E interacts with both antennas almost equally. The unique carbohydrate interaction explains the glycan-binding specificity and high affinity.
Phytohemagglutinin from Phaseolus vulgaris (PHA-E) displays a novel glycan recognition mode using a common legume lectin fold.,Nagae M, Soga K, Morita-Matsumoto K, Hanashima S, Ikeda A, Yamamoto K, Yamaguchi Y Glycobiology. 2014 Apr;24(4):368-78. doi: 10.1093/glycob/cwu004. Epub 2014 Jan, 16. PMID:24436051[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Nagae M, Soga K, Morita-Matsumoto K, Hanashima S, Ikeda A, Yamamoto K, Yamaguchi Y. Phytohemagglutinin from Phaseolus vulgaris (PHA-E) displays a novel glycan recognition mode using a common legume lectin fold. Glycobiology. 2014 Apr;24(4):368-78. doi: 10.1093/glycob/cwu004. Epub 2014 Jan, 16. PMID:24436051 doi:http://dx.doi.org/10.1093/glycob/cwu004
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