Tetracycline repressor protein

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<StructureSection load='2trt' size='350' side='right' scene='47/477771/Cv/1' caption='Tetracycline repressor protein complex with tetracycline and Mg+2 ion (green), [[2trt]]'>
<StructureSection load='2trt' size='350' side='right' scene='47/477771/Cv/1' caption='Tetracycline repressor protein complex with tetracycline and Mg+2 ion (green), [[2trt]]'>
== Function ==
== Function ==
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'''Tetracycline repressor protein''' (TetR) causes the resistance of bacterial cells to antibiotics like tetracycline (TC). TetR binds TC with higher affinity than the ribosome thus preventing TC from binding there and inhibiting the pathogenic bacteria protein synthesis<ref>PMID:21261817</ref>.
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'''Tetracycline repressor protein''' (TetR) causes the resistance of bacterial cells to antibiotics like tetracycline (TC). TetR binds TC with higher affinity than the ribosome thus preventing TC from binding there and inhibiting the pathogenic bacteria protein synthesis<ref>PMID:21261817</ref>. '''HTH-type TetR''' is a prokaryotic TetR which contains a DNA-binding '''H'''elix-'''T'''urn-'''H'''elix domain of ca. 60 residues<ref>PMID:8869638</ref>.
== Structural highlights ==
== Structural highlights ==
TetR binds DNA with a helix-turn-helix motif. The <scene name='47/477771/Cv/4'>inhibitor TC binds in the active site to several TetR side chains and to an octahedraly-coordinated Mg+2 ion</scene> <ref>PMID:8153629</ref>. Water molecules are shown as red spheres. <scene name='47/477771/Cv/5'>Mg coordination site</scene>.
TetR binds DNA with a helix-turn-helix motif. The <scene name='47/477771/Cv/4'>inhibitor TC binds in the active site to several TetR side chains and to an octahedraly-coordinated Mg+2 ion</scene> <ref>PMID:8153629</ref>. Water molecules are shown as red spheres. <scene name='47/477771/Cv/5'>Mg coordination site</scene>.
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</StructureSection>
 
== 3D Structures of tetracycline repressor protein==
== 3D Structures of tetracycline repressor protein==
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[[Tetracycline repressor protein 3D structures]]
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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</StructureSection>
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{{#tree:id=OrganizedByTopic|openlevels=0|
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*Tetracycline repressor protein class A
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**[[5mru]] – TetR + Mg + anhydro-TC - ''Pseudomonas''<br />
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*Tetracycline repressor protein class B
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**[[3fk6]] - EcTetR (mutant) <br />
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**[[3fk7]] - EcTetR (mutant) + Mg + 4-DDMA-ATC<br />
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**[[3zqf]], [[3zqg]], [[3zqh]], [[3zqi]] – EcTetR (mutant) + anti-inducer peptide<br />
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**[[2vkv]] – EcTetR (mutant) + Mg + anhydro-TC<br />
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**[[4ac0]] – EcTetR + Mg + minocycline<br />
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*Tetracycline repressor protein class D
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**[[4v2f]] – EcTetR D - ''Escherichia coli'' <br />
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**[[1bjz]], [[1a6i]], [[2ns7]], [[2xgc]], [[2xgd]], [[2xge]] - EcTetR (mutant) <br />
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**[[1qpi]] – EcTetR + DNA<br />
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*Tetracycline repressor protein class D complex with tetracycline and tetracycline derivative
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**[[2tct]] – EcTetR + Mg + chloro-TC<br />
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**[[4v2g]] – EcTetR + Mg + chloro-TC + iso- chloro-TC <br />
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**[[2trt]] – EcTetR + Mg + TC<br />
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**[[4b3a]] – EcTetR (mutant) + Mg + TC<br />
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**[[2x9d]], [[4b1r]] – EcTetR (mutant) + chloro-TC<br />
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**[[1ork]], [[5fkk]], [[5fkl]], [[5fkm]], [[5fkn]], [[5fko]] – EcTetR (mutant) + Mg + TC derivative<br />
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**[[2fj1]] – EcTetR (mutant) + Ni + chloro-TC<br />
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**[[2o7o]], [[2xrl]] – EcTetR (mutant) + Mg + doxy-TC<br />
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**[[2ns8]] – EcTetR (mutant) + Mg + TC peptide mimic<br />
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**[[2vke]] – EcTetR + Co + TC<br />
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**[[4d7m]] – EcTetR + Mg + anhydro-TC<br />
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**[[4d7n]] – EcTetR + K + anhydro-TC<br />
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**[[2xpu]] – EcTetR + anhydro-TC<br />
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**[[2xb5]] – EcTetR + Mg + iodo-TC<br />
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**[[2xpv]] – EcTetR + Mg + minocycline<br />
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**[[4abz]] – EcTetR + Mg + tigecycline<br />
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**[[2xpw]] – EcTetR + Mg + oxy-TC<br />
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**[[4aux]] – EcTetR + Mg + nitro-TC<br />
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**[[2x6o]] – EcTetR + TC derivative<br />
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*TetR class J
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**[[4d5c]] – TetR J – ''Proteus mirabilis''<br />
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*TetR class H
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**[[4d5f]] – TetR H – ''Mannheimia haemolytica''<br />
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*TetR family (transcriptional regulator)
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**[[1z0x]] – TetR – ''Enterococcus faecalis''<br />
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**[[1z77]], [[1zkg]], [[2id6]] – TetR – ''Thermotoga maritima''<br />
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**[[1zk8]], [[2fq4]] – TetR – ''Bacillus cereus''<br />
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**[[2g7s]] – TetR – ''Agrobacterium tumefaciens''<br />
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**[[2hyt]] – TetR – ''Pectobacterium atrosepticum''<br />
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**[[2qtq]] – TetR – ''Sphingobium aromaticivorans''<br />
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}}
 
== References ==
== References ==
<references/>
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

Tetracycline repressor protein complex with tetracycline and Mg+2 ion (green), 2trt

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References

  1. ↑ Bertram R, Hillen W. The application of Tet repressor in prokaryotic gene regulation and expression. Microb Biotechnol. 2008 Jan;1(1):2-16. doi: 10.1111/j.1751-7915.2007.00001.x. PMID:21261817 doi:http://dx.doi.org/10.1111/j.1751-7915.2007.00001.x
  2. ↑ Aramaki H, Yagi N, Suzuki M. Residues important for the function of a multihelical DNA binding domain in the new transcription factor family of Cam and Tet repressors. Protein Eng. 1995 Dec;8(12):1259-66. doi: 10.1093/protein/8.12.1259. PMID:8869638 doi:http://dx.doi.org/10.1093/protein/8.12.1259
  3. ↑ Hinrichs W, Kisker C, Duvel M, Muller A, Tovar K, Hillen W, Saenger W. Structure of the Tet repressor-tetracycline complex and regulation of antibiotic resistance. Science. 1994 Apr 15;264(5157):418-20. PMID:8153629

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

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