Thiaminase
From Proteopedia
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== Function == | == Function == | ||
| - | '''Thiaminase''' (Thi) cleaves vitamin B1. '''Thiaminase I''' catalyzes the elimination of the thiazole ring moiety from the thiamin through substitution of the methylene group with a nitrogenous base or sulfhydryl compound<ref>PMID:24351929</ref>. '''Thiaminase II''' catalyzes the hydrolysis of thiamin <ref>PMID:18028398</ref>. | + | '''Thiaminase''' (Thi) or '''thiamine pyridinylase''' cleaves vitamin B1. '''Thiaminase I''' catalyzes the elimination of the thiazole ring moiety from the thiamin through substitution of the methylene group with a nitrogenous base or sulfhydryl compound<ref>PMID:24351929</ref>. '''Thiaminase II''' catalyzes the hydrolysis of thiamin <ref>PMID:18028398</ref>. |
== Structural highlights == | == Structural highlights == | ||
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**[[2thi]], [[3thi]] – BtThia I – ''Bacillus thiaminolyticus'' <br /> | **[[2thi]], [[3thi]] – BtThia I – ''Bacillus thiaminolyticus'' <br /> | ||
**[[4thi]] – BtThia I + pyrimidine derivative <br /> | **[[4thi]] – BtThia I + pyrimidine derivative <br /> | ||
| + | **[[4kys]] – Thia I (mutant) + thiamin – ''Clostridium botulinum''<br /> | ||
* Thiaminase II | * Thiaminase II | ||
Current revision
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3D Structures of thiaminase
Updated on 25-November-2020
References
- ↑ Kreinbring CA, Remillard SP, Hubbard P, Brodkin HR, Leeper FJ, Hawksley D, Lai EY, Fulton C, Petsko GA, Ringe D. Structure of a eukaryotic thiaminase I. Proc Natl Acad Sci U S A. 2014 Jan 7;111(1):137-42. doi: 10.1073/pnas.1315882110., Epub 2013 Dec 18. PMID:24351929 doi:http://dx.doi.org/10.1073/pnas.1315882110
- ↑ Onozuka M, Konno H, Kawasaki Y, Akaji K, Nosaka K. Involvement of thiaminase II encoded by the THI20 gene in thiamin salvage of Saccharomyces cerevisiae. FEMS Yeast Res. 2008 Mar;8(2):266-75. Epub 2007 Nov 19. PMID:18028398 doi:http://dx.doi.org/10.1111/j.1567-1364.2007.00333.x
- ↑ Toms AV, Haas AL, Park JH, Begley TP, Ealick SE. Structural characterization of the regulatory proteins TenA and TenI from Bacillus subtilis and identification of TenA as a thiaminase II. Biochemistry. 2005 Feb 22;44(7):2319-29. PMID:15709744 doi:http://dx.doi.org/10.1021/bi0478648
