2vl6

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[[Image:2vl6.jpg|left|200px]]
 
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==STRUCTURAL ANALYSIS OF THE SULFOLOBUS SOLFATARICUS MCM PROTEIN N- TERMINAL DOMAIN==
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<StructureSection load='2vl6' size='340' side='right'caption='[[2vl6]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2vl6]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VL6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VL6 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_2vl6| PDB=2vl6 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vl6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vl6 OCA], [https://pdbe.org/2vl6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vl6 RCSB], [https://www.ebi.ac.uk/pdbsum/2vl6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vl6 ProSAT]</span></td></tr>
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</table>
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'''STRUCTURAL ANALYSIS OF THE SULFOLOBUS SOLFATARICUS MCM PROTEIN N-TERMINAL DOMAIN'''
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== Function ==
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[https://www.uniprot.org/uniprot/MCM_SACS2 MCM_SACS2] Presumptive replicative helicase. Has ATPase and DNA helicase activities. The latter preferentially melts 5'-tailed oligonucleotides and is stimulated by the SSB protein (single-stranded DNA binding protein). The active ATPase sites in the MCM ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The helicase function is proposed to use a partially sequential mode of ATP hydrolysis; the complex appears to tolerate multiple catalytically inactive subunits.<ref>PMID:11821426</ref>
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vl/2vl6_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vl6 ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
The Mini-Chromosome Maintenance (MCM) proteins are candidates of replicative DNA helicase in eukarya and archaea. Here we report a 2.8 A crystal structure of the N-terminal domain (residues 1-268) of the Sulfolobus solfataricus MCM (Sso MCM) protein. The structure reveals single-hexameric ring-like architecture, at variance from the protein of Methanothermobacter thermoautotrophicus (Mth). Moreover, the central channel in Sso MCM seems significantly narrower than the Mth counterpart, which appears to more favorably accommodate single-stranded DNA than double-stranded DNA, as supported by DNA-binding assays. Structural analysis also highlights the essential role played by the zinc-binding domain in the interaction with nucleic acids and allows us to speculate that the Sso MCM N-ter domain may function as a molecular clamp to grasp the single-stranded DNA passing through the central channel. On this basis possible DNA unwinding mechanisms are discussed.
The Mini-Chromosome Maintenance (MCM) proteins are candidates of replicative DNA helicase in eukarya and archaea. Here we report a 2.8 A crystal structure of the N-terminal domain (residues 1-268) of the Sulfolobus solfataricus MCM (Sso MCM) protein. The structure reveals single-hexameric ring-like architecture, at variance from the protein of Methanothermobacter thermoautotrophicus (Mth). Moreover, the central channel in Sso MCM seems significantly narrower than the Mth counterpart, which appears to more favorably accommodate single-stranded DNA than double-stranded DNA, as supported by DNA-binding assays. Structural analysis also highlights the essential role played by the zinc-binding domain in the interaction with nucleic acids and allows us to speculate that the Sso MCM N-ter domain may function as a molecular clamp to grasp the single-stranded DNA passing through the central channel. On this basis possible DNA unwinding mechanisms are discussed.
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==About this Structure==
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Structural analysis of the Sulfolobus solfataricus MCM protein N-terminal domain.,Liu W, Pucci B, Rossi M, Pisani FM, Ladenstein R Nucleic Acids Res. 2008 Jun;36(10):3235-43. Epub 2008 Apr 16. PMID:18417534<ref>PMID:18417534</ref>
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2VL6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VL6 OCA].
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==Reference==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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Structural analysis of the Sulfolobus solfataricus MCM protein N-terminal domain{dagger}, Liu W, Pucci B, Rossi M, Pisani FM, Ladenstein R, Nucleic Acids Res. 2008 Apr 16;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18417534 18417534]
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</div>
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[[Category: Single protein]]
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<div class="pdbe-citations 2vl6" style="background-color:#fffaf0;"></div>
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[[Category: Sulfolobus solfataricus]]
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== References ==
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[[Category: Ladenstein, R.]]
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<references/>
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[[Category: Liu, W.]]
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__TOC__
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[[Category: Pisani, F M.]]
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</StructureSection>
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[[Category: Pucci, B.]]
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[[Category: Large Structures]]
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[[Category: Rossi, M.]]
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[[Category: Saccharolobus solfataricus]]
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[[Category: Atp-binding]]
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[[Category: Ladenstein R]]
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[[Category: Dna binding protein]]
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[[Category: Liu W]]
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[[Category: Dna replication]]
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[[Category: Pisani FM]]
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[[Category: Dna-binding]]
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[[Category: Pucci B]]
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[[Category: Helicase]]
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[[Category: Rossi M]]
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[[Category: Hydrolase]]
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[[Category: Mcm]]
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[[Category: Nucleotide-binding]]
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[[Category: Ssdna binding]]
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[[Category: Zinc-finger]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 30 13:24:14 2008''
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Current revision

STRUCTURAL ANALYSIS OF THE SULFOLOBUS SOLFATARICUS MCM PROTEIN N- TERMINAL DOMAIN

PDB ID 2vl6

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