6u9j

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'''Unreleased structure'''
 
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The entry 6u9j is ON HOLD until Paper Publication
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==Crystal structure of ChuX==
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<StructureSection load='6u9j' size='340' side='right'caption='[[6u9j]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[6u9j]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U9J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6U9J FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6u9j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u9j OCA], [https://pdbe.org/6u9j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6u9j RCSB], [https://www.ebi.ac.uk/pdbsum/6u9j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6u9j ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q8X5N5_ECO57 Q8X5N5_ECO57]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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ChuW, ChuX, and ChuY are contiguous genes downstream from a single promoter that are expressed in the enteric pathogen Escherichia coli O157:H7 when iron is limiting. These genes, and the corresponding proteins, are part of a larger heme uptake and utilization operon that is common to several other enteric pathogens, such as Vibrio cholerae. The aerobic degradation of heme has been well characterized in humans and several pathogenic bacteria, including E. coli O157:H7, but only recently was it shown that ChuW catalyzes the anaerobic degradation of heme to release iron and produce a reactive tetrapyrrole termed "anaerobilin". ChuY has been shown to function as an anaerobilin reductase, in a role that parallels biliverdin reductase. In this work we have employed biochemical and biophysical approaches to further interrogate the mechanism of the anaerobic degradation of heme. We demonstrate that the iron atom of the heme does not participate in the catalytic mechanism of ChuW and that S-adenosyl-l-methionine binding induces conformational changes that favor catalysis. In addition, we show that ChuX and ChuY have synergistic and additive effects on the turnover rate of ChuW. Finally, we have found that ChuS is an effective source of heme or protoporphyrin IX for ChuW under anaerobic conditions. These data indicate that ChuS may have dual functionality in vivo. Specifically, ChuS serves as a heme oxygenase during aerobic metabolism of heme but functions as a cytoplasmic heme storage protein under anaerobic conditions, akin to what has been shown for PhuS (45% sequence identity) from Pseudomonas aeruginosa.
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Authors: Mathew, L.G., Lanzilotta, W.N.
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New Insight into the Mechanism of Anaerobic Heme Degradation.,Mathew LG, Beattie NR, Pritchett C, Lanzilotta WN Biochemistry. 2019 Nov 19;58(46):4641-4654. doi: 10.1021/acs.biochem.9b00841., Epub 2019 Nov 7. PMID:31652058<ref>PMID:31652058</ref>
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Description: Crystal structure of ChuX
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Lanzilotta, W.N]]
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<div class="pdbe-citations 6u9j" style="background-color:#fffaf0;"></div>
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[[Category: Mathew, L.G]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Escherichia coli O157:H7]]
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[[Category: Large Structures]]
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[[Category: Lanzilotta WN]]
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[[Category: Mathew LG]]

Current revision

Crystal structure of ChuX

PDB ID 6u9j

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