Tryptophan synthase
From Proteopedia
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<StructureSection load='' size='350' side='right' caption='Tryptophan synthase α subunit (yellow, salmon) and β subunit (cyan, green) of the α2β2 tetramer complex with pyridoxal-phosphate, glycerol-3-phosphate and Na+ ion (purple) [[1wbj]]' scene='52/525189/Cv/5'> | <StructureSection load='' size='350' side='right' caption='Tryptophan synthase α subunit (yellow, salmon) and β subunit (cyan, green) of the α2β2 tetramer complex with pyridoxal-phosphate, glycerol-3-phosphate and Na+ ion (purple) [[1wbj]]' scene='52/525189/Cv/5'> | ||
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== Function == | == Function == | ||
| - | '''Tryptophan synthase''' (TrpS) is an α2β2 tetramer participating in the biosynthesis of tryptophan<ref>PMID:11893063</ref>. TrpS '''α subunit''' catalyzes the formation of indole and glyceraldehyde-3-phosphate (G3P) from indole-3-glycerol phosphate (IGP). The '''β subunit''' catalyzes in a pyridoxal-phosphate (PLP) dependent reaction the formation of tryptophan (Trp) from indole and serine. The diffusion of indole from the α to the β subunit is facilitated via a hydrophobic channel connecting the subunits. | + | '''Tryptophan synthase''' (TrpS) is an α2β2 tetramer participating in the biosynthesis of tryptophan (Trp)<ref>PMID:11893063</ref>. TrpS '''α subunit''' catalyzes the formation of indole and glyceraldehyde-3-phosphate (G3P) from indole-3-glycerol phosphate (IGP). The '''β subunit''' catalyzes in a pyridoxal-phosphate (PLP) dependent reaction the formation of tryptophan (Trp) from indole and serine. The diffusion of indole from the α to the β subunit is facilitated via a hydrophobic channel connecting the subunits. '''Dimethylallyl tryptophan synthase''' (DMTS) catalyzes the alkylation of Trp by dimethylallyl phosphate to form 4-(γ,γ-dimethylallyl)-Trp<ref>PMID:1605639</ref>. |
== Relevance == | == Relevance == | ||
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== Structural highlights == | == Structural highlights == | ||
The <scene name='52/525189/Cv/6'>active site</scene> of TrpS subunit α contains the catalytic residues Glu and Asp<ref>PMID:16120446</ref>. Water molecules are shown as red spheres. | The <scene name='52/525189/Cv/6'>active site</scene> of TrpS subunit α contains the catalytic residues Glu and Asp<ref>PMID:16120446</ref>. Water molecules are shown as red spheres. | ||
| + | ==3D structures of tryptophan synthase== | ||
| + | [[Tryptophan synthase 3D structures]] | ||
</StructureSection> | </StructureSection> | ||
| - | ==3D structures of tryptophan synthase== | ||
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| - | Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | ||
| - | {{#tree:id=OrganizedByTopic|openlevels=0| | ||
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| - | *'''Tryptophan synthase α subunit''' | ||
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| - | **[[3vnd]] - TrpS α subunit – ''Shewanella frigidimarina''<br /> | ||
| - | **[[3tha]] - TrpS α subunit – ''Campylobacter jejuni''<br /> | ||
| - | **[[3nav]] - TrpS α subunit – ''Vibrio cholerae''<br /> | ||
| - | **[[1geq]] - PfTrpS α subunit - ''Pyrococcus furiosus''<br /> | ||
| - | **[[2dzp]], [[2dzs]], [[2dzt]], [[2dzu]], [[2dzv]], [[2dzw]], [[2dzx]], [[2e09]] - PfTrpS α subunit (mutant)<br /> | ||
| - | **[[1rd5]] - TrpS α subunit – corn<br /> | ||
| - | **[[1ujp]] - TtTrpS α subunit <br /> | ||
| - | **[[1v7y]], [[1wq5]], [[1xc4]] - EcTrpS α subunit <br /> | ||
| - | **[[1xcf]] - EcTrpS α subunit (mutant)<br /> | ||
| - | **[[2ekc]] - TrpS α subunit – ''Aquifex aeolicus''<br /> | ||
| - | **[[5kmy]], [[5k9x]] - TrpS α subunit – ''Legionella pneumophila''<br /> | ||
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| - | *Tryptophan synthase α binary complex | ||
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| - | **[[1wxj]] - TtTrpS α subunit + indole propanol phosphate<br /> | ||
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| - | *'''Tryptophan synthase β subunit''' | ||
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| - | **[[1x1q]] - TtTrpS β subunit – ''Thermus thermophilus''<br /> | ||
| - | **[[2dh6]] - EcTrpS β subunit - ''Escherichia coli''<br /> | ||
| - | **[[2o2e]], [[2o2j]] - MtTrpS β subunit – ''Mycobacterium tuberculosis''<br /> | ||
| - | **[[1v8z]] - PfTrpS β1 subunit <br /> | ||
| - | **[[4neg]] - TrpS β subunit – ''Bacillus anthracis''<br /> | ||
| - | **[[4qys]] - SsTrpS β2 subunit – ''Sulfolobus solfataricus''<br /> | ||
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| - | *Tryptophan synthase β complex | ||
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| - | **[[2dh5]] - EcTrpS β subunit + PLP<br /> | ||
| - | **[[6eqn]] - SsTrpS β2 subunit + PLP<br /> | ||
| - | **[[5dvz]] - PfTrpS β1 subunit + PLP derivative <br /> | ||
| - | **[[6am7]], [[6amc]], [[6cuv]] - PfTrpS β1 subunit (mutant) + PLP derivative <br /> | ||
| - | **[[5dw0]] - PfTrpS β1 subunit + Ser derivative <br /> | ||
| - | **[[5dw3]] - PfTrpS β1 subunit + PLP derivative + Trp<br /> | ||
| - | **[[6am8]], [[6ami]] - PfTrpS β1 subunit (mutant) + PLP derivative + Trp<br /> | ||
| - | **[[5t6m]] - PfTrpS β1 subunit + PLP derivative + Trp derivative<br /> | ||
| - | **[[5vm5]] - PfTrpS β1 subunit + PLP derivative + Ser derivative<br /> | ||
| - | **[[5ixj]] - PfTrpS β1 subunit + PLP derivative + Thr<br /> | ||
| - | **[[6am9]], [[6amh]] - PfTrpS β1 subunit (mutant) + PLP-Ser derivative <br /> | ||
| - | **[[6cut]] - PfTrpS β1 subunit (mutant) + Trp analog <br /> | ||
| - | **[[6cuz]] - PfTrpS β1 subunit (mutant) + PLP derivative + ethylserine<br /> | ||
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| - | *'''Tryptophan synthase α+β subunits''' | ||
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| - | **[[1wdw]] - PfTrpS α + β subunits <br /> | ||
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| - | *Tryptophan synthase α+β binary complex | ||
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| - | **[[1ttp]], [[1ttq]], [[1bks]], [[2wsy]], [[1kfk]] - StTrpS α + β + PLP – ''Salmonella typhimurium''<br /> | ||
| - | **[[1a5a]], [[1k8x]] - StTrpS α (mutant) + β + PLP<br /> | ||
| - | **[[1k7x]], [[2j9z]] - StTrpS α + β (mutant) + PLP<br /> | ||
| - | **[[2tys]] - StTrpS α (mutant) + β (mutant) + Trp derivative<br /> | ||
| - | **[[1kfe]] - StTrpS α (mutant) + β + pyridoxal-serine-monophsphate<br /> | ||
| - | **[[1kfj]] - StTrpS α + β + pyridoxal-serine-monophsphate<br /> | ||
| - | **[[2j9y]] - StTrpS α + β (mutant) + enoic acid derivative<br /> | ||
| - | **[[3pr2]], [[4hn4]], [[4hpj]], [[4hpx]], [[4ht3]], [[4kkx]] - StTrpS α + β + inhibitor<br /> | ||
| - | **[[5kzm]] - TrpS α + β + PLP derivative – ''Francisella tularensis''<br /> | ||
| - | **[[5kin]] - TrpS α + β + PLP derivative – ''Streptococcus pneumoniae''<br /> | ||
| - | **[[5e0k]] - PfTrpS α + β + PLP derivative <br /> | ||
| - | **[[5ocw]], [[5tcg]], [[5tci]], [[5tcj]] - MtTrpS α + β + inhibitor<br /> | ||
| - | **[[5tcf]] - MtTrpS α + β + PLP derivative <br /> | ||
| - | **[[5tch]] - MtTrpS α + β (mutant) + PLP derivative <br /> | ||
| - | **[[5e0k]] - PfTrpS α + β + PLP derivative <br /> | ||
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| - | *Tryptophan synthase α+β ternary complex with PLP and inhibitor | ||
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| - | **[[1a50]], [[1cw2]], [[1c9d]], [[1cx9]], [[1c8v]], [[1c29]], [[2cle]], [[2clh]], [[2cli]], [[2clk]], [[2cll]], [[2clm]], [[2clo]], [[2clf]], [[5bw6]], [[4zqc]], [[4y6g]], [[4xug]], [[4wx2]], [[5bw6]] - StTrpS α + β + inhibitor + PLP<br /> | ||
| - | **[[5cgq]] - StTrpS α + β + inhibitor + PLP + Trp<br /> | ||
| - | **[[1fuy]] - StTrpS α + β (mutant) + inhibitor + PLP<br /> | ||
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| - | *Tryptophan synthase α+β ternary complex with PLP and IGP derivatives | ||
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| - | **[[1a5b]], [[1kfb]] - StTrpS α (mutant) + β + IGP + PLP<br /> | ||
| - | **[[1k8z]] - StTrpS α + β (mutant) + IGP derivative + PLP<br /> | ||
| - | **[[1kfc]] - StTrpS α (mutant) + β + IGP derivative + PLP<br /> | ||
| - | **[[1k3u]], [[1k7e]], [[1k7f]] - StTrpS α + β + IGP derivative + PLP<br /> | ||
| - | **[[1qoq]], [[2rh9]], [[2rhg]] - StTrpS α + β + IGP + PLP<br /> | ||
| - | **[[1qop]] - StTrpS α + β + IGP analog + PLP<br /> | ||
| - | **[[2trs]], [[1beu]] - StTrpS α (mutant) + β (mutant) + pyridoxal-serine-monophsphate + IGP analog | ||
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| - | *Tryptophan synthase α+β ternary complex with PLP and G3P derivatives | ||
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| - | **[[1wbj]] - StTrpS α + β + G3P + PLP<br /> | ||
| - | **[[2j9x]] - StTrpS α + β + G3P + acrylic acid derivative<br /> | ||
| - | **[[1k8y]] - StTrpS α + β (mutant) + G3P derivative + PLP<br /> | ||
| - | **[[1tjp]] - StTrpS α + β + G3P derivative + PLP<br /> | ||
| - | **[[2tsy]] - StTrpS α (mutant) + β (mutant) + pyridoxal-serine-monophsphate + G3P | ||
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| - | **[[1ubs]] - StTrpS α (mutant) + β (mutant) + serine + PLP<br /> | ||
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| - | *Tryptophan synthase α+β quaternary complex | ||
| - | **[[1a5s]] - StTrpS α + β + serine + inhibitor + PLP<br /> | ||
| - | **[[5cgq]] - StTrpS α + β + Trp + inhibitor + PLP<br /> | ||
| - | **[[3cep]] - StTrpS α + β + G3P + indoline + PLP<br /> | ||
| - | **[[6du1]], [[6dua]], [[6dwe]], [[6e9p]] - MtTrpS α + β + malonate + inhibitor + PLP derivative<br /> | ||
| - | }} | ||
== References == | == References == | ||
<references/> | <references/> | ||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||
Current revision
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References
- ↑ Miles EW. Tryptophan synthase: a multienzyme complex with an intramolecular tunnel. Chem Rec. 2001;1(2):140-51. PMID:11893063
- ↑ Gebler JC, Poulter CD. Purification and characterization of dimethylallyl tryptophan synthase from Claviceps purpurea. Arch Biochem Biophys. 1992 Jul;296(1):308-13. PMID:1605639 doi:10.1016/0003-9861(92)90577-j
- ↑ Shen H, Yang Y, Wang F, Zhang Y, Ye N, Xu S, Wang H. Characterization of the putative tryptophan synthase beta-subunit from Mycobacterium tuberculosis. Acta Biochim Biophys Sin (Shanghai). 2009 May;41(5):379-88. PMID:19430702
- ↑ Kulik V, Hartmann E, Weyand M, Frey M, Gierl A, Niks D, Dunn MF, Schlichting I. On the structural basis of the catalytic mechanism and the regulation of the alpha subunit of tryptophan synthase from Salmonella typhimurium and BX1 from maize, two evolutionarily related enzymes. J Mol Biol. 2005 Sep 23;352(3):608-20. PMID:16120446 doi:10.1016/j.jmb.2005.07.014
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