2orx

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[[Image:2orx.gif|left|200px]]
 
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==Structural Basis for Ligand Binding and Heparin Mediated Activation of Neuropilin==
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The line below this paragraph, containing "STRUCTURE_2orx", creates the "Structure Box" on the page.
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<StructureSection load='2orx' size='340' side='right'caption='[[2orx]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2orx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ORX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ORX FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2orx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2orx OCA], [https://pdbe.org/2orx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2orx RCSB], [https://www.ebi.ac.uk/pdbsum/2orx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2orx ProSAT]</span></td></tr>
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{{STRUCTURE_2orx| PDB=2orx | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NRP1_RAT NRP1_RAT] Receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, the PLGF-2 isoform of PGF, the VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis (By similarity).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/or/2orx_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2orx ConSurf].
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<div style="clear:both"></div>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Neuropilin (Nrp) is a cell surface receptor with essential roles in angiogenesis and axon guidance. Interactions between Nrp and the positively charged C termini of its ligands, VEGF and semaphorin, are mediated by Nrp domains b1 and b2, which share homology to coagulation factor domains. We report here the crystal structure of the tandem b1 and b2 domains of Nrp-1 (N1b1b2) and show that they form a single structural unit. Cocrystallization of N1b1b2 with Tuftsin, a peptide mimic of the VEGF C terminus, reveals the site of interaction with the basic tail of VEGF on the b1 domain. We also show that heparin promotes N1b1b2 dimerization and map the heparin binding site on N1b1b2. These results provide a detailed picture of interactions at the core of the Nrp signaling complex and establish a molecular basis for the synergistic effects of heparin on Nrp-mediated signaling.
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'''Structural Basis for Ligand Binding and Heparin Mediated Activation of Neuropilin'''
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Structural basis for ligand and heparin binding to neuropilin B domains.,Vander Kooi CW, Jusino MA, Perman B, Neau DB, Bellamy HD, Leahy DJ Proc Natl Acad Sci U S A. 2007 Apr 10;104(15):6152-7. Epub 2007 Apr 3. PMID:17405859<ref>PMID:17405859</ref>
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==Overview==
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Neuropilin (Nrp) is a cell surface receptor with essential roles in angiogenesis and axon guidance. Interactions between Nrp and the positively charged C termini of its ligands, VEGF and semaphorin, are mediated by Nrp domains b1 and b2, which share homology to coagulation factor domains. We report here the crystal structure of the tandem b1 and b2 domains of Nrp-1 (N1b1b2) and show that they form a single structural unit. Cocrystallization of N1b1b2 with Tuftsin, a peptide mimic of the VEGF C terminus, reveals the site of interaction with the basic tail of VEGF on the b1 domain. We also show that heparin promotes N1b1b2 dimerization and map the heparin binding site on N1b1b2. These results provide a detailed picture of interactions at the core of the Nrp signaling complex and establish a molecular basis for the synergistic effects of heparin on Nrp-mediated signaling.
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==About this Structure==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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2ORX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ORX OCA].
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</div>
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<div class="pdbe-citations 2orx" style="background-color:#fffaf0;"></div>
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==Reference==
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==See Also==
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Structural basis for ligand and heparin binding to neuropilin B domains., Vander Kooi CW, Jusino MA, Perman B, Neau DB, Bellamy HD, Leahy DJ, Proc Natl Acad Sci U S A. 2007 Apr 10;104(15):6152-7. Epub 2007 Apr 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17405859 17405859]
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*[[Neuropilin|Neuropilin]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Single protein]]
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[[Category: Bellamy HD]]
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[[Category: Bellamy, H D.]]
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[[Category: Jusino MA]]
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[[Category: Jusino, M A.]]
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[[Category: Leahy DJ]]
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[[Category: Kooi, C W.Vander.]]
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[[Category: Neau DB]]
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[[Category: Leahy, D J.]]
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[[Category: Perman B]]
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[[Category: Neau, D B.]]
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[[Category: Vander Kooi CW]]
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[[Category: Perman, B.]]
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[[Category: Membrane protein]]
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[[Category: Neuropilin]]
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[[Category: Signaling protein]]
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[[Category: Tuftsin]]
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[[Category: Vegf]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 30 13:30:04 2008''
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Current revision

Structural Basis for Ligand Binding and Heparin Mediated Activation of Neuropilin

PDB ID 2orx

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