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| | <StructureSection load='6r2n' size='340' side='right'caption='[[6r2n]], [[Resolution|resolution]] 2.60Å' scene=''> | | <StructureSection load='6r2n' size='340' side='right'caption='[[6r2n]], [[Resolution|resolution]] 2.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6r2n]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R2N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6R2N FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6r2n]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R2N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6R2N FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.596Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucokinase Glucokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.2 2.7.1.2] </span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6r2n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r2n OCA], [http://pdbe.org/6r2n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6r2n RCSB], [http://www.ebi.ac.uk/pdbsum/6r2n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6r2n ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6r2n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r2n OCA], [https://pdbe.org/6r2n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6r2n RCSB], [https://www.ebi.ac.uk/pdbsum/6r2n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6r2n ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/HXKG_KLULA HXKG_KLULA]] Glukokinase specific for aldohexoses. Phosphorylates glucose and mannose, but not fructose.<ref>PMID:17573926</ref> | + | [https://www.uniprot.org/uniprot/HXKG_KLULA HXKG_KLULA] Glukokinase specific for aldohexoses. Phosphorylates glucose and mannose, but not fructose.<ref>PMID:17573926</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 6r2n" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 6r2n" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Hexokinase 3D structures|Hexokinase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Glucokinase]] | + | [[Category: Kluyveromyces lactis]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Grudnik, P]] | + | [[Category: Grudnik P]] |
| - | [[Category: Wator, E]] | + | [[Category: Wator E]] |
| - | [[Category: Zak, K]] | + | [[Category: Zak K]] |
| - | [[Category: Kluyveromyces lacti]]
| + | |
| - | [[Category: Sugar metabolism]]
| + | |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
Function
HXKG_KLULA Glukokinase specific for aldohexoses. Phosphorylates glucose and mannose, but not fructose.[1]
Publication Abstract from PubMed
Glucose phosphorylating enzymes are crucial in the regulation of basic cellular processes, including metabolism and gene expression. Glucokinases and hexokinases provide a pool of phosphorylated glucose in an adenosine diphosphate (ADP)- and ATP-dependent manner to shape the cell metabolism. The glucose processing enzymes from Kluyveromyces lactis are poorly characterized despite the emerging contribution of this yeast strain to industrial and laboratory scale biotechnology. The first reports on K. lactis glucokinase (KlGlk1) positioned the enzyme as an essential component required for glucose signaling. Nevertheless, no biochemical and structural information was available until now. Here, we present the first crystal structure of KlGlk1 together with biochemical characterization, including substrate specificity and enzyme kinetics. Additionally, comparative analysis of the presented structure and the prior structures of lactis hexokinase (KlHxk1) demonstrates the potential transitions between open and closed enzyme conformations upon ligand binding.
Crystal Structure of Kluyveromyces lactis Glucokinase (KlGlk1).,Zak KM, Kalinska M, Wator E, Kuska K, Krutyholowa R, Dubin G, Popowicz GM, Grudnik P Int J Mol Sci. 2019 Sep 28;20(19). pii: ijms20194821. doi: 10.3390/ijms20194821. PMID:31569356[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Kettner K, Muller EC, Otto A, Rodel G, Breunig KD, Kriegel TM. Identification and characterization of a novel glucose-phosphorylating enzyme in Kluyveromyces lactis. FEMS Yeast Res. 2007 Aug;7(5):683-92. doi: 10.1111/j.1567-1364.2007.00259.x. Epub, 2007 Jun 16. PMID:17573926 doi:http://dx.doi.org/10.1111/j.1567-1364.2007.00259.x
- ↑ Zak KM, Kalinska M, Wator E, Kuska K, Krutyholowa R, Dubin G, Popowicz GM, Grudnik P. Crystal Structure of Kluyveromyces lactis Glucokinase (KlGlk1). Int J Mol Sci. 2019 Sep 28;20(19). pii: ijms20194821. doi: 10.3390/ijms20194821. PMID:31569356 doi:http://dx.doi.org/10.3390/ijms20194821
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