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5frv

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crystal structure of the phenol-responsive sensory domain of the transcription activator PoxR in complex with 4-methylphenol (Cresol)

Structural highlights

5frv is a 2 chain structure with sequence from Cupriavidus necator. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.9Å
Ligands:	,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

O84957_9RALS

Publication Abstract from PubMed

Positive phenol-degradative gene regulator (PoxR) is a sigma(54)-dependent AAA+ ATPase transcription activator that regulates the catabolism of phenols. The PoxR sensory domain detects phenols and relays signals for the activation of transcription. Here we report the first structure of the phenol sensory domain bound to phenol and five derivatives. It exists as a tightly intertwined homodimer with a phenol-binding pocket buried inside, placing two C termini on the same side of the dimer. His102 and Trp130 interact with the hydroxyl group of the phenol in a cavity surrounded by rigid hydrophobic residues on one side and a flexible region on the other. Each monomer has a V4R fold with a unique zinc-binding site. A shift at the C-terminal helix suggests that there is a possible conformational change upon ligand binding. The results provide a structural basis of chemical effector binding for transcriptional regulation with broad implications for protein engineering.

Structural Analysis of the Phenol-Responsive Sensory Domain of the Transcription Activator PoxR.,Patil VV, Park KH, Lee SG, Woo E Structure. 2016 Apr 5;24(4):624-30. doi: 10.1016/j.str.2016.03.006. PMID:27050690^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Patil VV, Park KH, Lee SG, Woo E. Structural Analysis of the Phenol-Responsive Sensory Domain of the Transcription Activator PoxR. Structure. 2016 Apr 5;24(4):624-30. doi: 10.1016/j.str.2016.03.006. PMID:27050690 doi:http://dx.doi.org/10.1016/j.str.2016.03.006

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5frv"

Categories: Cupriavidus necator | Large Structures | Patil VV | Woo EJ

@@ Line 3: / Line 3: @@
 <StructureSection load='5frv' size='340' side='right'caption='[[5frv]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5frv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_17697_[[alcaligenes_eutrophus]] Atcc 17697 [[alcaligenes eutrophus]]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FRV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5FRV FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5frv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cupriavidus_necator Cupriavidus necator]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5FRV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5FRV FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCR:P-CRESOL'>PCR</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5fru|5fru]], [[5frw|5frw]], [[5frx|5frx]], [[5fry|5fry]], [[5frz|5frz]], [[5fs0|5fs0]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PCR:P-CRESOL'>PCR</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5frv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5frv OCA], [http://pdbe.org/5frv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5frv RCSB], [http://www.ebi.ac.uk/pdbsum/5frv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5frv ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5frv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5frv OCA], [https://pdbe.org/5frv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5frv RCSB], [https://www.ebi.ac.uk/pdbsum/5frv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5frv ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/O84957_9RALS O84957_9RALS]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 23: @@
 __TOC__
 </StructureSection>
+[[Category: Cupriavidus necator]]
 [[Category: Large Structures]]
-[[Category: Patil, V V]]
+[[Category: Patil VV]]
-[[Category: Woo, E J]]
+[[Category: Woo EJ]]
-[[Category: Activator]]
-[[Category: Atpase family]]
-[[Category: Dmpr]]
-[[Category: Enhancer-binding protein]]
-[[Category: Phenol]]
-[[Category: Poxr]]
-[[Category: Transcription]]

5frv

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Current revision

crystal structure of the phenol-responsive sensory domain of the transcription activator PoxR in complex with 4-methylphenol (Cresol)

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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