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| | ==Structure of human soluble guanylate cyclase in the NO activated state== | | ==Structure of human soluble guanylate cyclase in the NO activated state== |
| - | <StructureSection load='6jt2' size='340' side='right'caption='[[6jt2]], [[Resolution|resolution]] 3.80Å' scene=''> | + | <SX load='6jt2' size='340' side='right' viewer='molstar' caption='[[6jt2]], [[Resolution|resolution]] 3.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6jt2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JT2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JT2 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6jt2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JT2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JT2 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G2P:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>G2P</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.8Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GUCY1A1, GUC1A3, GUCSA3, GUCY1A3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), GUCY1B1, GUC1B3, GUCSB3, GUCY1B3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G2P:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>G2P</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Guanylate_cyclase Guanylate cyclase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.6.1.2 4.6.1.2] </span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jt2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jt2 OCA], [https://pdbe.org/6jt2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jt2 RCSB], [https://www.ebi.ac.uk/pdbsum/6jt2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jt2 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6jt2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jt2 OCA], [http://pdbe.org/6jt2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jt2 RCSB], [http://www.ebi.ac.uk/pdbsum/6jt2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jt2 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | == Disease == | + | == Function == |
| - | [[http://www.uniprot.org/uniprot/GCYA1_HUMAN GCYA1_HUMAN]] Moyamoya disease with early-onset achalasia. The disease is caused by mutations affecting the gene represented in this entry. | + | [https://www.uniprot.org/uniprot/GCYB1_HUMAN GCYB1_HUMAN] |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | Soluble guanylate cyclase (sGC) is the primary sensor of nitric oxide. It has a central role in nitric oxide signalling and has been implicated in many essential physiological processes and disease conditions. The binding of nitric oxide boosts the enzymatic activity of sGC. However, the mechanism by which nitric oxide activates the enzyme is unclear. Here we report the cryo-electron microscopy structures of the human sGCalpha1beta1 heterodimer in different functional states. These structures revealed that the transducer module bridges the nitric oxide sensor module and the catalytic module. Binding of nitric oxide to the beta1 haem-nitric oxide and oxygen binding (H-NOX) domain triggers the structural rearrangement of the sensor module and a conformational switch of the transducer module from bending to straightening. The resulting movement of the N termini of the catalytic domains drives structural changes within the catalytic module, which in turn boost the enzymatic activity of sGC.
| + | |
| | | | |
| - | Structural insights into the mechanism of human soluble guanylate cyclase.,Kang Y, Liu R, Wu JX, Chen L Nature. 2019 Oct;574(7777):206-210. doi: 10.1038/s41586-019-1584-6. Epub 2019 Sep, 12. PMID:31514202<ref>PMID:31514202</ref>
| + | ==See Also== |
| - | | + | *[[Guanylate cyclase 3D structures|Guanylate cyclase 3D structures]] |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6jt2" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| - | </StructureSection> | + | </SX> |
| - | [[Category: Guanylate cyclase]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Human]]
| + | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Chen, L]] | + | [[Category: Chen L]] |
| - | [[Category: Kang, Y]] | + | [[Category: Kang Y]] |
| - | [[Category: Liu, R]] | + | [[Category: Liu R]] |
| - | [[Category: Wu, J X]] | + | [[Category: Wu J-X]] |
| - | [[Category: Signaling protein]]
| + | |
| - | [[Category: Soluble guanylate cyclase]]
| + | |
